Overview
| Uniprot ID | O14734 |
|---|
| Protein Name | Acyl-coenzyme A thioesterase 8 |
|---|
| Gene Name | ACOT8 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 52 |
RHYWVPAKRLFGGQI |
Function
Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:15194431, PubMed:9153233, PubMed:9299485). Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:9153233, PubMed:9299485). Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). Is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431)
Protein Sequence
10
MSSPQAPEDG
20
QGCGDRGDPP
30
GDLRSVLVTT
40
VLNLEPLDED
50
LFRGRHYWVP
60
AKRLFGGQIV
70
GQALVAAAKS
80
VSEDVHVHSL
90
HCYFVRAGDP
100
KLPVLYQVER
110
TRTGSSFSVR
120
SVKAVQHGKP
130
IFICQASFQQ
140
AQPSPMQHQF
150
SMPTVPPPEE
160
LLDCETLIDQ
170
YLRDPNLQKR
180
YPLALNRIAA
190
QEVPIEIKPV
200
NPSPLSQLQR
210
MEPKQMFWVR
220
ARGYIGEGDM
230
KMHCCVAAYI
240
SDYAFLGTAL
250
LPHQWQHKVH
260
FMVSLDHSMW
270
FHAPFRADHW
280
MLYECESPWA
290
GGSRGLVHGR
300
LWRQDGVLAV
310
TCAQEGVIRV
KPQVSESKL
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0006633 |
fatty acid biosynthetic process |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005782 |
peroxisomal matrix |
| Molecular Function |
GO:0047603 |
acetoacetyl-CoA hydrolase activity |
| Molecular Function |
GO:0003986 |
acetyl-CoA hydrolase activity |
| Molecular Function |
GO:0016289 |
acyl-CoA hydrolase activity |
| Molecular Function |
GO:0052689 |
carboxylic ester hydrolase activity |
| Molecular Function |
GO:0033882 |
choloyl-CoA hydrolase activity |
| Molecular Function |
GO:0047617 |
fatty acyl-CoA hydrolase activity |
| Molecular Function |
GO:0047994 |
hydroxymethylglutaryl-CoA hydrolase activity |
| Molecular Function |
GO:0052816 |
long-chain fatty acyl-CoA hydrolase activity |
| Molecular Function |
GO:0052815 |
medium-chain fatty acyl-CoA hydrolase activity |
| Molecular Function |
GO:0004778 |
succinyl-CoA hydrolase activity |
| Biological Process |
GO:0006637 |
acyl-CoA metabolic process |
| Biological Process |
GO:0036109 |
alpha-linolenic acid metabolic process |
| Biological Process |
GO:0006699 |
bile acid biosynthetic process |
| Biological Process |
GO:0043649 |
dicarboxylic acid catabolic process |
| Biological Process |
GO:0033540 |
fatty acid beta-oxidation using acyl-CoA oxidase |
| Biological Process |
GO:0009062 |
fatty acid catabolic process |
| Biological Process |
GO:0010561 |
negative regulation of glycoprotein biosynthetic process |
| Biological Process |
GO:0016559 |
peroxisome fission |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.