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Overview

Uniprot IDO14745
Protein NameNa(+)/H(+) exchange regulatory cofactor NHE-RF1
Gene NameNHERF1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
172 GFNLHSDKSKPGQFI
32 GFHLHGEKGKLGQYI
34 HLHGEKGKLGQYIRL
50 EPGSPAEKAGLLAGD

Function

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa

Protein Sequence

10 MSADAAAGAP 20 LPRLCCLEKG 30 PNGYGFHLHG 40 EKGKLGQYIR 50 LVEPGSPAEK 60 AGLLAGDRLV 70 EVNGENVEKE 80 THQQVVSRIR 90 AALNAVRLLV 100 VDPETDEQLQ 110 KLGVQVREEL 120 LRAQEAPGQA 130 EPPAAAEVQG 140 AGNENEPREA 150 DKSHPEQREL 160 RPRLCTMKKG 170 PSGYGFNLHS 180 DKSKPGQFIR 190 SVDPDSPAEA 200 SGLRAQDRIV 210 EVNGVCMEGK 220 QHGDVVSAIR 230 AGGDETKLLV 240 VDRETDEFFK 250 KCRVIPSQEH 260 LNGPLPVPFT 270 NGEIQKENSR 280 EALAEAALES 290 PRPALVRSAS 300 SDTSEELNSQ 310 DSPPKQDSTA 320 PSSTSSSDPI 330 LDFNISLAMA 340 KERAHQKRSS 350 KRAPQMDWSK KNELFSNL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Biological Process GO:0045930 negative regulation of mitotic cell cycle
Biological Process GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Process GO:0010642 negative regulation of platelet-derived growth factor receptor signaling pathway
Biological Process GO:0007097 nuclear migration
Biological Process GO:0007009 plasma membrane organization
Biological Process GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
Biological Process GO:0072659 protein localization to plasma membrane
Biological Process GO:0065003 protein-containing complex assembly
Biological Process GO:0008360 regulation of cell shape
Biological Process GO:0008361 regulation of cell size
Biological Process GO:0045859 regulation of protein kinase activity
Biological Process GO:0070293 renal absorption
Biological Process GO:0097291 renal phosphate ion absorption
Biological Process GO:0150104 transport across blood-brain barrier
Biological Process GO:0016055 Wnt signaling pathway
Cellular Component GO:0016324 apical plasma membrane
Cellular Component GO:0031526 brush border membrane
Cellular Component GO:0071944 cell periphery
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0012505 endomembrane system
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0030175 filopodium
Cellular Component GO:0016020 membrane
Cellular Component GO:0005902 microvillus
Cellular Component GO:0031528 microvillus membrane
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0098797 plasma membrane protein complex
Cellular Component GO:0001726 ruffle
Cellular Component GO:0097225 sperm midpiece
Cellular Component GO:0032426 stereocilium tip
Cellular Component GO:0031982 vesicle
Molecular Function GO:0031698 beta-2 adrenergic receptor binding
Molecular Function GO:0008013 beta-catenin binding
Molecular Function GO:0099103 channel activator activity
Molecular Function GO:0017081 chloride channel regulator activity
Molecular Function GO:0050780 dopamine receptor binding
Molecular Function GO:0070851 growth factor receptor binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0030165 PDZ domain binding
Molecular Function GO:0019902 phosphatase binding
Molecular Function GO:0043495 protein-membrane adaptor activity
Molecular Function GO:0005102 signaling receptor binding
Molecular Function GO:0031799 type 2 metabotropic glutamate receptor binding
Molecular Function GO:0031800 type 3 metabotropic glutamate receptor binding
Biological Process GO:0032782 bile acid secretion
Biological Process GO:0090660 cerebrospinal fluid circulation
Biological Process GO:0044782 cilium organization
Biological Process GO:0045198 establishment of epithelial cell apical/basal polarity
Biological Process GO:0051683 establishment of Golgi localization
Biological Process GO:0051939 gamma-aminobutyric acid import
Biological Process GO:0022612 gland morphogenesis
Biological Process GO:0034635 glutathione transport
Biological Process GO:0098739 import across plasma membrane
Biological Process GO:0045199 maintenance of epithelial cell apical/basal polarity
Biological Process GO:0030033 microvillus assembly
Biological Process GO:0002009 morphogenesis of an epithelium
Biological Process GO:0090090 negative regulation of canonical Wnt signaling pathway
Biological Process GO:2000146 negative regulation of cell motility
Biological Process GO:0008285 negative regulation of cell population proliferation
Biological Process GO:0070373 negative regulation of ERK1 and ERK2 cascade

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.