Search Results

Overview

Uniprot IDO14777
Protein NameKinetochore protein NDC80 homolog
Gene NameNDC80
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
26 LRSQDVNKQGLYTPQ
59 RKVSLFGKRTSGHGS

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912, PubMed:9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:23891108, PubMed:25743205)

Protein Sequence

10 MKRSSVSSGG 20 AGRLSMQELR 30 SQDVNKQGLY 40 TPQTKEKPTF 50 GKLSINKPTS 60 ERKVSLFGKR 70 TSGHGSRNSQ 80 LGIFSSSEKI 90 KDPRPLNDKA 100 FIQQCIRQLC 110 EFLTENGYAH 120 NVSMKSLQAP 130 SVKDFLKIFT 140 FLYGFLCPSY 150 ELPDTKFEEE 160 VPRIFKDLGY 170 PFALSKSSMY 180 TVGAPHTWPH 190 IVAALVWLID 200 CIKIHTAMKE 210 SSPLFDDGQP 220 WGEETEDGIM 230 HNKLFLDYTI 240 KCYESFMSGA 250 DSFDEMNAEL 260 QSKLKDLFNV 270 DAFKLESLEA 280 KNRALNEQIA 290 RLEQEREKEP 300 NRLESLRKLK 310 ASLQGDVQKY 320 QAYMSNLESH 330 SAILDQKLNG 340 LNEEIARVEL 350 ECETIKQENT 360 RLQNIIDNQK 370 YSVADIERIN 380 HERNELQQTI 390 NKLTKDLEAE 400 QQKLWNEELK 410 YARGKEAIET 420 QLAEYHKLAR 430 KLKLIPKGAE 440 NSKGYDFEIK 450 FNPEAGANCL 460 VKYRAQVYVP 470 LKELLNETEE 480 EINKALNKKM 490 GLEDTLEQLN 500 AMITESKRSV 510 RTLKEEVQKL 520 DDLYQQKIKE 530 AEEEDEKCAS 540 ELESLEKHKH 550 LLESTVNQGL 560 SEAMNELDAV 570 QREYQLVVQT 580 TTEERRKVGN 590 NLQRLLEMVA 600 THVGSVEKHL 610 EEQIAKVDRE 620 YEECMSEDLS 630 ENIKEIRDKY 640 EKKATLIKSS EE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000940 outer kinetochore
Molecular Function GO:0030332 cyclin binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0140483 kinetochore adaptor activity
Molecular Function GO:0008017 microtubule binding
Biological Process GO:0051315 attachment of mitotic spindle microtubules to kinetochore
Biological Process GO:0008608 attachment of spindle microtubules to kinetochore
Biological Process GO:0051301 cell division
Biological Process GO:0051298 centrosome duplication
Biological Process GO:0007059 chromosome segregation
Biological Process GO:0000132 establishment of mitotic spindle orientation
Biological Process GO:0008315 G2/MI transition of meiotic cell cycle
Biological Process GO:0051310 metaphase chromosome alignment
Biological Process GO:0000278 mitotic cell cycle
Biological Process GO:0000070 mitotic sister chromatid segregation
Biological Process GO:0007094 mitotic spindle assembly checkpoint signaling
Biological Process GO:0007052 mitotic spindle organization
Biological Process GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
Biological Process GO:0007057 spindle assembly involved in female meiosis I
Cellular Component GO:0000775 chromosome, centromeric region
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0016020 membrane
Cellular Component GO:0031262 Ndc80 complex
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.