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Overview

Uniprot IDO14818
Protein NameProteasome subunit alpha type-7
Gene NamePSMA7
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
115 TRYIASLKQRYTQSN
157 SGTYHAWKANAIGRG
28 YAQEAVKKGSTAVGV
47 IVVLGVEKKSVAKLQ
52 VEKKSVAKLQDERTV

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response

Protein Sequence

10 MSYDRAITVF 20 SPDGHLFQVE 30 YAQEAVKKGS 40 TAVGVRGRDI 50 VVLGVEKKSV 60 AKLQDERTVR 70 KICALDDNVC 80 MAFAGLTADA 90 RIVINRARVE 100 CQSHRLTVED 110 PVTVEYITRY 120 IASLKQRYTQ 130 SNGRRPFGIS 140 ALIVGFDFDG 150 TPRLYQTDPS 160 GTYHAWKANA 170 IGRGAKSVRE 180 FLEKNYTDEA 190 IETDDLTIKL 200 VIKALLEVVQ 210 SGGKNIELAV 220 MRRDQSLKIL 230 NPEEIEKYVA 240 EIEKEKEENE KKKQKKAS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000502 proteasome complex
Cellular Component GO:0005839 proteasome core complex
Cellular Component GO:0019773 proteasome core complex, alpha-subunit complex
Cellular Component GO:0034515 proteasome storage granule
Cellular Component GO:0008021 synaptic vesicle
Molecular Function GO:0042802 identical protein binding
Biological Process GO:0006915 apoptotic process
Biological Process GO:0043374 CD8-positive, alpha-beta T cell differentiation
Biological Process GO:0160165 CD8-positive, alpha-beta T cell homeostasis
Biological Process GO:0071357 cellular response to type I interferon
Biological Process GO:0006974 DNA damage response
Biological Process GO:0006281 DNA repair
Biological Process GO:0030317 flagellated sperm motility
Biological Process GO:0002376 immune system process
Biological Process GO:0045590 negative regulation of regulatory T cell differentiation
Biological Process GO:0032743 positive regulation of interleukin-2 production
Biological Process GO:0032760 positive regulation of tumor necrosis factor production
Biological Process GO:0032729 positive regulation of type II interferon production
Biological Process GO:0010498 proteasomal protein catabolic process
Biological Process GO:0010499 proteasomal ubiquitin-independent protein catabolic process
Biological Process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
Biological Process GO:2000045 regulation of G1/S transition of mitotic cell cycle
Biological Process GO:0061136 regulation of proteasomal protein catabolic process
Biological Process GO:0006979 response to oxidative stress
Biological Process GO:0034341 response to type II interferon
Biological Process GO:0045063 T-helper 1 cell differentiation
Biological Process GO:0072539 T-helper 17 cell differentiation
Biological Process GO:0045061 thymic T cell selection

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.

[9] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.