Search Results

Overview

Uniprot IDO14832
Protein NamePhytanoyl-CoA dioxygenase, peroxisomal
Gene NamePHYH
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
108 MRDVTISKSEYAPSE
116 SEYAPSEKMITKVQD
120 PSEKMITKVQDFQED
128 VQDFQEDKELFRYCT
218 GTHKGSLKPHDYPKW
276 NKTQGFRKAISCHFA
302 TSQENIEKEVVGIAH

Function

Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms) (PubMed:10744784, PubMed:12031666, PubMed:12923223, PubMed:9326939). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs (PubMed:10744784, PubMed:12923223)

Protein Sequence

10 MEQLRAAARL 20 QIVLGHLGRP 30 SAGAVVAHPT 40 SGTISSASFH 50 PQQFQYTLDN 60 NVLTLEQRKF 70 YEENGFLVIK 80 NLVPDADIQR 90 FRNEFEKICR 100 KEVKPLGLTV 110 MRDVTISKSE 120 YAPSEKMITK 130 VQDFQEDKEL 140 FRYCTLPEIL 150 KYVECFTGPN 160 IMAMHTMLIN 170 KPPDSGKKTS 180 RHPLHQDLHY 190 FPFRPSDLIV 200 CAWTAMEHIS 210 RNNGCLVVLP 220 GTHKGSLKPH 230 DYPKWEGGVN 240 KMFHGIQDYE 250 ENKARVHLVM 260 EKGDTVFFHP 270 LLIHGSGQNK 280 TQGFRKAISC 290 HFASADCHYI 300 DVKGTSQENI 310 EKEVVGIAHK 320 FFGAENSVNL 330 KDIWMFRARL VKGERTNL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0097731 9+0 non-motile cilium
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005782 peroxisomal matrix
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0031406 carboxylic acid binding
Molecular Function GO:0008198 ferrous iron binding
Molecular Function GO:0031418 L-ascorbic acid binding
Molecular Function GO:0048244 phytanoyl-CoA dioxygenase activity
Biological Process GO:0019606 2-oxobutyrate catabolic process
Biological Process GO:0006103 2-oxoglutarate metabolic process
Biological Process GO:0001561 fatty acid alpha-oxidation
Biological Process GO:0006720 isoprenoid metabolic process
Biological Process GO:0097089 methyl-branched fatty acid metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.