Search Results

Overview

Uniprot IDO14974
Protein NameProtein phosphatase 1 regulatory subunit 12A
Gene NamePPP1R12A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
320 SQKTFKNKETLIIEP
413 TPTSPIKKFPTTATK
420 KFPTTATKISPKEEE
442 TWRLGLRKTGSYGAL
532 SSSYTRRKWEDDLKK
551 NEGSTYHKSCSFGRR

Function

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity

Protein Sequence

10 MKMADAKQKR 20 NEQLKRWIGS 30 ETDLEPPVVK 40 RQKTKVKFDD 50 GAVFLAACSS 60 GDTDEVLKLL 70 HRGADINYAN 80 VDGLTALHQA 90 CIDDNVDMVK 100 FLVENGANIN 110 QPDNEGWIPL 120 HAAASCGYLD 130 IAEFLIGQGA 140 HVGAVNSEGD 150 TPLDIAEEEA 160 MEELLQNEVN 170 RQGVDIEAAR 180 KEEERIMLRD 190 ARQWLNSGHI 200 NDVRHAKSGG 210 TALHVAAAKG 220 YTEVLKLLIQ 230 AGYDVNIKDY 240 DGWTPLHAAA 250 HWGKEEACRI 260 LVDNLCDMEM 270 VNKVGQTAFD 280 VADEDILGYL 290 EELQKKQNLL 300 HSEKRDKKSP 310 LIESTANMDN 320 NQSQKTFKNK 330 ETLIIEPEKN 340 ASRIESLEQE 350 KVDEEEEGKK 360 DESSCSSEED 370 EEDDSESEAE 380 TDKTKPLASV 390 TNANTSSTQA 400 APVAVTTPTV 410 SSGQATPTSP 420 IKKFPTTATK 430 ISPKEEERKD 440 ESPATWRLGL 450 RKTGSYGALA 460 EITASKEGQK 470 EKDTAGVTRS 480 ASSPRLSSSL 490 DNKEKEKDSK 500 GTRLAYVAPT 510 IPRRLASTSD 520 IEEKENRDSS 530 SLRTSSSYTR 540 RKWEDDLKKN 550 SSVNEGSTYH 560 KSCSFGRRQD 570 DLISSSVPST 580 TSTPTVTSAA 590 GLQKSLLSST 600 STTTKITTGS 610 SSAGTQSSTS 620 NRLWAEDSTE 630 KEKDSVPTAV 640 TIPVAPTVVN 650 AAASTTTLTT 660 TTAGTVSSTT 670 EVRERRRSYL 680 TPVRDEESES 690 QRKARSRQAR 700 QSRRSTQGVT 710 LTDLQEAEKT 720 IGRSRSTRTR 730 EQENEEKEKE 740 EKEKQDKEKQ 750 EEKKESETSR 760 EDEYKQKYSR 770 TYDETYQRYR 780 PVSTSSSTTP 790 SSSLSTMSSS 800 LYASSQLNRP 810 NSLVGITSAY 820 SRGITKENER 830 EGEKREEEKE 840 GEDKSQPKSI 850 RERRRPREKR 860 RSTGVSFWTQ 870 DSDENEQEQQ 880 SDTEEGSNKK 890 ETQTDSISRY 900 ETSSTSAGDR 910 YDSLLGRSGS 920 YSYLEERKPY 930 SSRLEKDDST 940 DFKKLYEQIL 950 AENEKLKAQL 960 HDTNMELTDL 970 KLQLEKATQR 980 QERFADRSLL 990 EMEKRERRAL 1000 ERRISEMEEE 1010 LKMLPDLKAD 1020 NQRLKDENGA 1030 LIRVISKLSK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0031672 A band
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005813 centrosome
Cellular Component GO:0043292 contractile muscle fiber
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0072357 PTW/PP1 phosphatase complex
Cellular Component GO:0001725 stress fiber
Cellular Component GO:0030018 Z disc
Molecular Function GO:0071889 14-3-3 protein binding
Molecular Function GO:0004857 enzyme inhibitor activity
Molecular Function GO:0017020 myosin phosphatase regulator activity
Molecular Function GO:0019208 phosphatase regulator activity
Molecular Function GO:0019901 protein kinase binding
Biological Process GO:0007098 centrosome cycle
Biological Process GO:0000278 mitotic cell cycle
Biological Process GO:0043086 negative regulation of catalytic activity
Biological Process GO:0048812 neuron projection morphogenesis
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0006470 protein dephosphorylation
Biological Process GO:0030155 regulation of cell adhesion
Biological Process GO:0007165 signal transduction

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.