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Overview

Uniprot IDO15145
Protein NameActin-related protein 2/3 complex subunit 3
Gene NameARPC3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
158 PQNDKPSKWWTCFVK
165 KWWTCFVKRQFMNKS
29 LPIRSQFKGPAPRET
61 FFKNYEIKNEADRTL

Function

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947)

Protein Sequence

10 MPAYHSSLMD 20 PDTKLIGNMA 30 LLPIRSQFKG 40 PAPRETKDTD 50 IVDEAIYYFK 60 ANVFFKNYEI 70 KNEADRTLIY 80 ITLYISECLK 90 KLQKCNSKSQ 100 GEKEMYTLGI 110 TNFPIPGEPG 120 FPLNAIYAKP 130 ANKQEDEVMR 140 AYLQQLRQET 150 GLRLCEKVFD 160 PQNDKPSKWW 170 TCFVKRQFMN KSLSGPGQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005885 Arp2/3 protein complex
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0030027 lamellipodium
Cellular Component GO:0016020 membrane
Cellular Component GO:0005634 nucleus
Cellular Component GO:0035861 site of double-strand break
Molecular Function GO:0003779 actin binding
Molecular Function GO:0005200 structural constituent of cytoskeleton
Biological Process GO:0070358 actin polymerization-dependent cell motility
Biological Process GO:0034314 Arp2/3 complex-mediated actin nucleation
Biological Process GO:0030833 regulation of actin filament polymerization
Biological Process GO:1900242 regulation of synaptic vesicle endocytosis

Reference

[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.