Search Results

Overview

Uniprot IDO15156
Protein NameZinc finger and BTB domain-containing protein 7B
Gene NameZBTB7B
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
206 RRSRKPRKAFLQTKG
212 RKAFLQTKGARANHL
360 KIIHGAGKLPRHMRT

Function

Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Exerts distinct biological functions in the mammary epithelial cells and T cells in a tissue-specific manner. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Cross-antagonism between ZBTB7B and CBF complexes are determinative to CD4 versus CD8 cell fate decision. Suppresses RUNX3 expression and imposes CD4+ lineage fate by inducing the SOCS suppressors of cytokine signaling. induces, as a transcriptional activator, SOCS genes expression which represses RUNX3 expression and promotes the CD4+ lineage fate. During CD4 lineage commitment, associates with multiple sites at the CD8 locus, acting as a negative regulator of the CD8 promoter and enhancers by epigenetic silencing through the recruitment of class II histone deacetylases, such as HDAC4 and HDAC5, to these loci. Regulates the development of IL17-producing CD1d-restricted naural killer (NK) T cells. Also functions as an important metabolic regulator in the lactating mammary glands. Critical feed-forward regulator of insulin signaling in mammary gland lactation, directly regulates expression of insulin receptor substrate-1 (IRS-1) and insulin-induced Akt-mTOR-SREBP signaling (By similarity). Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes (PubMed:9370309). Potent driver of brown fat development, thermogenesis and cold-induced beige fat formation. Recruits the brown fat lncRNA 1 (Blnc1):HNRNPU ribonucleoprotein complex to activate thermogenic gene expression in brown and beige adipocytes (By similarity)

Protein Sequence

10 MGSPEDDLIG 20 IPFPDHSSEL 30 LSCLNEQRQL 40 GHLCDLTIRT 50 QGLEYRTHRA 60 VLAACSHYFK 70 KLFTEGGGGA 80 VMGAGGSGTA 90 TGGAGAGVCE 100 LDFVGPEALG 110 ALLEFAYTAT 120 LTTSSANMPA 130 VLQAARLLEI 140 PCVIAACMEI 150 LQGSGLEAPS 160 PDEDDCERAR 170 QYLEAFATAT 180 ASGVPNGEDS 190 PPQVPLPPPP 200 PPPPRPVARR 210 SRKPRKAFLQ 220 TKGARANHLV 230 PEVPTVPAHP 240 LTYEEEEVAG 250 RVGSSGGSGP 260 GDSYSPPTGT 270 ASPPEGPQSY 280 EPYEGEEEEE 290 ELVYPPAYGL 300 AQGGGPPLSP 310 EELGSDEDAI 320 DPDLMAYLSS 330 LHQDNLAPGL 340 DSQDKLVRKR 350 RSQMPQECPV 360 CHKIIHGAGK 370 LPRHMRTHTG 380 EKPFACEVCG 390 VRFTRNDKLK 400 IHMRKHTGER 410 PYSCPHCPAR 420 FLHSYDLKNH 430 MHLHTGDRPY 440 ECHLCHKAFA 450 KEDHLQRHLK 460 GQNCLEVRTR 470 RRRKDDAPPH 480 YPPPSTAAAS 490 PAGLDLSNGH 500 LDTFRLSLAR 510 FWEQSAPTGP 520 PVSTPGPPDD 530 DEEEGAPTTP QAEGAMESS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
Molecular Function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function GO:0001217 DNA-binding transcription repressor activity
Molecular Function GO:0042826 histone deacetylase binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:1990837 sequence-specific double-stranded DNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:1990845 adaptive thermogenesis
Biological Process GO:0007398 ectoderm development
Biological Process GO:0007595 lactation
Biological Process GO:0043377 negative regulation of CD8-positive, alpha-beta T cell differentiation
Biological Process GO:0051141 negative regulation of NK T cell proliferation
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0001865 NK T cell differentiation
Biological Process GO:0090336 positive regulation of brown fat cell differentiation
Biological Process GO:0043372 positive regulation of CD4-positive, alpha-beta T cell differentiation
Biological Process GO:0120162 positive regulation of cold-induced thermogenesis
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:0046628 positive regulation of insulin receptor signaling pathway
Biological Process GO:0032740 positive regulation of interleukin-17 production
Biological Process GO:2000640 positive regulation of SREBP signaling pathway
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0032868 response to insulin
Biological Process GO:0006366 transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.