Overview
| Uniprot ID | O15318 |
|---|
| Protein Name | DNA-directed RNA polymerase III subunit RPC7 |
|---|
| Gene Name | POLR3G |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 131 |
KKAKDAGKGTPLTNT |
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:33558764, PubMed:34675218, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:20154270, PubMed:20413673, PubMed:35637192). Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. At resting state, occupies the active site of apo Pol III and keeps Pol III in an autoinhibitory mode, preventing non-specific transcription (PubMed:33558764, PubMed:33558766, PubMed:35637192). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370)
Protein Sequence
10
MAGNKGRGRA
20
AYTFNIEAVG
30
FSKGEKLPDV
40
VLKPPPLFPD
50
TDYKPVPLKT
60
GEGEEYMLAL
70
KQELRETMKR
80
MPYFIETPEE
90
RQDIERYSKR
100
YMKVYKEEWI
110
PDWRRLPREM
120
MPRNKCKKAG
130
PKPKKAKDAG
140
KGTPLTNTED
150
VLKKMEELEK
160
RGDGEKSDEE
170
NEEKEGSKEK
180
SKEGDDDDDD
190
DAAEQEEYDE
200
EEQEEENDYI
210
NSYFEDGDDF
220
GADSDDNMDE
ATY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016604 |
nuclear body |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005666 |
RNA polymerase III complex |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0003899 |
DNA-directed RNA polymerase activity |
| Biological Process |
GO:0008283 |
cell population proliferation |
| Biological Process |
GO:0051607 |
defense response to virus |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0045089 |
positive regulation of innate immune response |
| Biological Process |
GO:0032728 |
positive regulation of interferon-beta production |
| Biological Process |
GO:0006359 |
regulation of transcription by RNA polymerase III |
| Biological Process |
GO:0006386 |
termination of RNA polymerase III transcription |
| Biological Process |
GO:0006383 |
transcription by RNA polymerase III |
| Biological Process |
GO:0006384 |
transcription initiation at RNA polymerase III promoter |
Reference
PMID: N/A