Overview
| Uniprot ID | O15382 |
|---|
| Protein Name | Branched-chain-amino-acid aminotransferase, mitochondrial |
|---|
| Gene Name | BCAT2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 321 |
VERTITMKQLLRALE |
| 377 |
LRFQKELKEIQYGIR |
| 44 |
LQLEMTQKPHKKPGP |
Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:17050531, PubMed:25653144, PubMed:8702755). Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes (By similarity). Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning (By similarity). May also function as a transporter of branched chain alpha-keto acids (By similarity)
Protein Sequence
10
MAAAALGQIW
20
ARKLLSVPWL
30
LCGPRRYASS
40
SFKAADLQLE
50
MTQKPHKKPG
60
PGEPLVFGKT
70
FTDHMLMVEW
80
NDKGWGQPRI
90
QPFQNLTLHP
100
ASSSLHYSLQ
110
LFEGMKAFKG
120
KDQQVRLFRP
130
WLNMDRMLRS
140
AMRLCLPSFD
150
KLELLECIRR
160
LIEVDKDWVP
170
DAAGTSLYVR
180
PVLIGNEPSL
190
GVSQPTRALL
200
FVILCPVGAY
210
FPGGSVTPVS
220
LLADPAFIRA
230
WVGGVGNYKL
240
GGNYGPTVLV
250
QQEALKRGCE
260
QVLWLYGPDH
270
QLTEVGTMNI
280
FVYWTHEDGV
290
LELVTPPLNG
300
VILPGVVRQS
310
LLDMAQTWGE
320
FRVVERTITM
330
KQLLRALEEG
340
RVREVFGSGT
350
ACQVCPVHRI
360
LYKDRNLHIP
370
TMENGPELIL
380
RFQKELKEIQ
390
YGIRAHEWMF
PV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0004084 |
branched-chain-amino-acid transaminase activity |
| Molecular Function |
GO:0052656 |
L-isoleucine-2-oxoglutarate transaminase activity |
| Molecular Function |
GO:0052654 |
L-leucine-2-oxoglutarate transaminase activity |
| Molecular Function |
GO:0052655 |
L-valine-2-oxoglutarate transaminase activity |
| Biological Process |
GO:0009082 |
branched-chain amino acid biosynthetic process |
| Biological Process |
GO:0009083 |
branched-chain amino acid catabolic process |
| Biological Process |
GO:0050873 |
brown fat cell differentiation |
| Biological Process |
GO:0097009 |
energy homeostasis |
| Biological Process |
GO:0009098 |
L-leucine biosynthetic process |
| Biological Process |
GO:0009099 |
L-valine biosynthetic process |
| Biological Process |
GO:0006629 |
lipid metabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.