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Overview

Uniprot IDO15511
Protein NameActin-related protein 2/3 complex subunit 5
Gene NameARPC5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
13 VSSARFRKVDVDEYD
3 *****MSKNTVSSAR
67 KNPPINTKSQAVKDR

Function

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947)

Protein Sequence

10 MSKNTVSSAR 20 FRKVDVDEYD 30 ENKFVDEEDG 40 GDGQAGPDEG 50 EVDSCLRQGN 60 MTAALQAALK 70 NPPINTKSQA 80 VKDRAGSIVL 90 KVLISFKAND 100 IEKAVQSLDK 110 NGVDLLMKYI 120 YKGFESPSDN 130 SSAMLLQWHE 140 KALAAGGVGS 150 IVRVLTARKT V

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005885 Arp2/3 protein complex
Cellular Component GO:0030863 cortical cytoskeleton
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:1904813 ficolin-1-rich granule lumen
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0030027 lamellipodium
Cellular Component GO:0005634 nucleus
Cellular Component GO:0034774 secretory granule lumen
Cellular Component GO:0035861 site of double-strand break
Molecular Function GO:0003779 actin binding
Molecular Function GO:0030674 protein-macromolecule adaptor activity
Molecular Function GO:0005200 structural constituent of cytoskeleton
Biological Process GO:0030036 actin cytoskeleton organization
Biological Process GO:0034314 Arp2/3 complex-mediated actin nucleation
Biological Process GO:0016477 cell migration
Biological Process GO:0030833 regulation of actin filament polymerization

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.