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Overview

Uniprot IDO15519
Protein NameCASP8 and FADD-like apoptosis regulator
Gene NameCFLAR
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
117 SSLIFLMKDYMGRGK
124 KDYMGRGKISKEKSF

Function

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells (PubMed:31046799). Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity

Protein Sequence

10 MSAEVIHQVE 20 EALDTDEKEM 30 LLFLCRDVAI 40 DVVPPNVRDL 50 LDILRERGKL 60 SVGDLAELLY 70 RVRRFDLLKR 80 ILKMDRKAVE 90 THLLRNPHLV 100 SDYRVLMAEI 110 GEDLDKSDVS 120 SLIFLMKDYM 130 GRGKISKEKS 140 FLDLVVELEK 150 LNLVAPDQLD 160 LLEKCLKNIH 170 RIDLKTKIQK 180 YKQSVQGAGT 190 SYRNVLQAAI 200 QKSLKDPSNN 210 FRLHNGRSKE 220 QRLKEQLGAQ 230 QEPVKKSIQE 240 SEAFLPQSIP 250 EERYKMKSKP 260 LGICLIIDCI 270 GNETELLRDT 280 FTSLGYEVQK 290 FLHLSMHGIS 300 QILGQFACMP 310 EHRDYDSFVC 320 VLVSRGGSQS 330 VYGVDQTHSG 340 LPLHHIRRMF 350 MGDSCPYLAG 360 KPKMFFIQNY 370 VVSEGQLEDS 380 SLLEVDGPAM 390 KNVEFKAQKR 400 GLCTVHREAD 410 FFWSLCTADM 420 SLLEQSHSSP 430 SLYLQCLSQK 440 LRQERKRPLL 450 DLHIELNGYM 460 YDWNSRVSAK 470 EKYYVWLQHT 480 LRKKLILSYT

Gene Ontology

Classification GO ID Description
Cellular Component GO:0031265 CD95 death-inducing signaling complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0031264 death-inducing signaling complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0097342 ripoptosome
Molecular Function GO:0004197 cysteine-type endopeptidase activity
Molecular Function GO:0005123 death receptor binding
Molecular Function GO:0008047 enzyme activator activity
Molecular Function GO:0002020 protease binding
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0006915 apoptotic process
Biological Process GO:0071549 cellular response to dexamethasone stimulus
Biological Process GO:0071364 cellular response to epidermal growth factor stimulus
Biological Process GO:0071392 cellular response to estradiol stimulus
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0071732 cellular response to nitric oxide
Biological Process GO:0008625 extrinsic apoptotic signaling pathway via death domain receptors
Biological Process GO:0030225 macrophage differentiation
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0010667 negative regulation of cardiac muscle cell apoptotic process
Biological Process GO:1903845 negative regulation of cellular response to transforming growth factor beta stimulus
Biological Process GO:2001237 negative regulation of extrinsic apoptotic signaling pathway
Biological Process GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
Biological Process GO:1903944 negative regulation of hepatocyte apoptotic process
Biological Process GO:1901740 negative regulation of myoblast fusion
Biological Process GO:1903427 negative regulation of reactive oxygen species biosynthetic process
Biological Process GO:0043123 positive regulation of canonical NF-kappaB signal transduction
Biological Process GO:0070374 positive regulation of ERK1 and ERK2 cascade
Biological Process GO:1903055 positive regulation of extracellular matrix organization
Biological Process GO:0072126 positive regulation of glomerular mesangial cell proliferation
Biological Process GO:2000347 positive regulation of hepatocyte proliferation
Biological Process GO:0043525 positive regulation of neuron apoptotic process
Biological Process GO:0010976 positive regulation of neuron projection development
Biological Process GO:0006508 proteolysis
Biological Process GO:0060544 regulation of necroptotic process
Biological Process GO:0014842 regulation of skeletal muscle satellite cell proliferation
Biological Process GO:0033574 response to testosterone
Biological Process GO:0014732 skeletal muscle atrophy
Biological Process GO:0007519 skeletal muscle tissue development
Biological Process GO:0043403 skeletal muscle tissue regeneration
Biological Process GO:0014866 skeletal myofibril assembly

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.