Search Results
Overview
| Uniprot ID | O35143 |
|---|---|
| Protein Name | ATPase inhibitor, mitochondrial |
| Gene Name | Atp5if1 |
| Organism | Mus musculus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 103 | KKKIQQLKNNH**** |
| 49 | EAGGAFGKREKAEED |
| 64 | RYFREKTKEQLAALR |
| 83 | DEIDHHSKEIERLQK |
| 90 | KEIERLQKQIERHKK |
Function
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (PubMed:23135403)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0009986 | cell surface |
| Biological Process | GO:1901030 | positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway |
| Biological Process | GO:1903052 | positive regulation of proteolysis involved in protein catabolic process |
| Biological Process | GO:1905091 | positive regulation of type 2 mitophagy |
| Biological Process | GO:0072593 | reactive oxygen species metabolic process |
| Biological Process | GO:1903578 | regulation of ATP metabolic process |
| Biological Process | GO:1903214 | regulation of protein targeting to mitochondrion |
| Biological Process | GO:0002931 | response to ischemia |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005739 | mitochondrion |
| Cellular Component | GO:0032991 | protein-containing complex |
| Molecular Function | GO:0043532 | angiostatin binding |
| Molecular Function | GO:0051117 | ATPase binding |
| Molecular Function | GO:0042030 | ATPase inhibitor activity |
| Molecular Function | GO:0005516 | calmodulin binding |
| Molecular Function | GO:0019899 | enzyme binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0140260 | mitochondrial proton-transporting ATP synthase complex binding |
| Biological Process | GO:0030218 | erythrocyte differentiation |
| Biological Process | GO:0006783 | heme biosynthetic process |
| Biological Process | GO:0051882 | mitochondrial depolarization |
| Biological Process | GO:0010667 | negative regulation of cardiac muscle cell apoptotic process |
| Biological Process | GO:0001937 | negative regulation of endothelial cell proliferation |
| Biological Process | GO:0051346 | negative regulation of hydrolase activity |
| Biological Process | GO:1905707 | negative regulation of mitochondrial ATP synthesis coupled proton transport |
Reference
[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.
[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.