Search Results

Overview

Uniprot ID	O35459
Protein Name	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Gene Name	Ech1
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
150	DLISKYQKTFTVIEK
230	ELTFSARKMMADEAL
316	SVQAAMEKRDTKSIT
76	VQLNRPEKRNAMNRA
97	ECFQKISKDSDCRAV

Function

Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids. Together with the 2,4-dienoyl-CoA reductase (DECR) and the Enoyl-CoA delta isomerase 1 (ECI1) they allow reentrance of the enoyl-CoA into the beta-oxidation cycle (By similarity). Able to isomerize 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position (By similarity). Based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (By similarity)

Protein Sequence

10 MATAMTVSSK 20 LRGLLMQQLR 30 GTSQLYFNIS 40 LRSLSSSAQE 50 ASKRAPEEVS 60 DHNYESIQVT 70 SAQKHVLHVQ 80 LNRPEKRNAM 90 NRAFWRELVE 100 CFQKISKDSD 110 CRAVVVSGAG 120 KMFTSGIDLM 130 DMASELMQPS 140 GDDAARIAWY 150 LRDLISKYQK 160 TFTVIEKCPK 170 PVIAAIHGGC 180 IGGGVDLVSA 190 CDIRYCTQDA 200 FFQIKEVDMG 210 LAADVGTLQR 220 LPKVIGNQSL 230 VNELTFSARK 240 MMADEALDSG 250 LVSRVFQDKD 260 AMLNAAFALA 270 ADISSKSPVA 280 VQGSKINLIY 290 SRDHSVDESL 300 DYMATWNMSM 310 LQTQDIIKSV 320 QAAMEKRDTK SITFSKL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005777	peroxisome
Molecular Function	GO:0051750	delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity
Biological Process	GO:0006635	fatty acid beta-oxidation

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.