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Overview

Uniprot IDO35857
Protein NameMitochondrial import inner membrane translocase subunit TIM44
Gene NameTimm44
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
121 TSEAIKKKLGELTGT
405 VMVIKNSKGEVYDGD

Function

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (PubMed:31618756). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity)

Protein Sequence

10 MAAAALRGGW 20 CRCPRRCLGS 30 GIQFLSSHNL 40 PHGSSYQISR 50 PGRELTLTKS 60 YSSGSRKGFL 70 SGLLDNIKQE 80 LAKNKEMKES 90 IKKFRDEAKK 100 LEESDALQEA 110 RRKYKSIESE 120 TVRTSEAIKK 130 KLGELTGTVK 140 ESLDEVSKSD 150 LGRKIKEGVE 160 EAARTAKQSA 170 ESVSKSGEKL 180 GKTAAFKAIS 190 QGVESVKKEL 200 DESVLGQTGP 210 YRRPERLRKR 220 TEFAGAKFKE 230 SKVFEANEEA 240 LGVVLHKDSK 250 WYQQWKDFKD 260 NNVVFNRFFE 270 MKMKYDESDN 280 VLIRASRALT 290 DKVTDLLGGL 300 FSKTEMSEVL 310 TEILRVDPTF 320 DKDHFLHQCE 330 TDIIPNILEA 340 MISGELDILK 350 DWCYEATYSQ 360 LAHPIQQAKA 370 LGFQFHSRIL 380 DISNVDLAMG 390 KMMEQGPVLI 400 VTFQAQVVMV 410 IKNSKGEVYD 420 GDPDKVQRML 430 YVWALCRDQE 440 ELNPYAAWRL 450 LDISASSTEQ IL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005744 TIM23 mitochondrial import inner membrane translocase complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0051087 protein-folding chaperone binding
Biological Process GO:0006886 intracellular protein transport
Biological Process GO:0030150 protein import into mitochondrial matrix

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.