Search Results

Overview

Uniprot IDO43143
Protein NameATP-dependent RNA helicase DHX15
Gene NameDHX15
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
17 GEDYPSGKKRAGTDG
18 EDYPSGKKRAGTDGK
185 MRSLPGPKRGVACTQ
25 KRAGTDGKDRDRDRD
3 *****MSKRHRLDLG
407 IFEPPPPKKQNGAIG
408 FEPPPPKKQNGAIGR
515 GSVVLQLKKLGIDDL
516 SVVLQLKKLGIDDLV
786 QLDRIIAKLQSKEYS

Function

RNA helicase involved in mRNA processing and antiviral innate immunity (PubMed:19103666, PubMed:19432882, PubMed:24782566, PubMed:24990078, PubMed:32179686, PubMed:34161762). Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA (PubMed:19103666). In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns (PubMed:19103666). Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome (PubMed:24782566, PubMed:24990078, PubMed:34161762). Acts as an RNA virus sensor: recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3) (PubMed:24782566, PubMed:24990078, PubMed:34161762). Involved in intestinal antiviral innate immunity together with NLRP6: recognizes and binds viral dsRNA and promotes activation of the NLRP6 inflammasome in intestinal epithelial cells to restrict infection by enteric viruses (PubMed:34161762). The NLRP6 inflammasome acts by promoting maturation and secretion of IL18 in the extracellular milieu (PubMed:34161762). Also involved in antibacterial innate immunity by promoting Wnt-induced antimicrobial protein expression in Paneth cells (By similarity)

Protein Sequence

10 MSKRHRLDLG 20 EDYPSGKKRA 30 GTDGKDRDRD 40 RDREDRSKDR 50 DRERDRGDRE 60 REREKEKEKE 70 LRASTNAMLI 80 SAGLPPLKAS 90 HSAHSTHSAH 100 STHSTHSAHS 110 THAGHAGHTS 120 LPQCINPFTN 130 LPHTPRYYDI 140 LKKRLQLPVW 150 EYKDRFTDIL 160 VRHQSFVLVG 170 ETGSGKTTQI 180 PQWCVEYMRS 190 LPGPKRGVAC 200 TQPRRVAAMS 210 VAQRVADEMD 220 VMLGQEVGYS 230 IRFEDCSSAK 240 TILKYMTDGM 250 LLREAMNDPL 260 LERYGVIILD 270 EAHERTLATD 280 ILMGVLKEVV 290 RQRSDLKVIV 300 MSATLDAGKF 310 QIYFDNCPLL 320 TIPGRTHPVE 330 IFYTPEPERD 340 YLEAAIRTVI 350 QIHMCEEEEG 360 DLLLFLTGQE 370 EIDEACKRIK 380 REVDDLGPEV 390 GDIKIIPLYS 400 TLPPQQQQRI 410 FEPPPPKKQN 420 GAIGRKVVVS 430 TNIAETSLTI 440 DGVVFVIDPG 450 FAKQKVYNPR 460 IRVESLLVTA 470 ISKASAQQRA 480 GRAGRTRPGK 490 CFRLYTEKAY 500 KTEMQDNTYP 510 EILRSNLGSV 520 VLQLKKLGID 530 DLVHFDFMDP 540 PAPETLMRAL 550 ELLNYLAALN 560 DDGDLTELGS 570 MMAEFPLDPQ 580 LAKMVIASCD 590 YNCSNEVLSI 600 TAMLSVPQCF 610 VRPTEAKKAA 620 DEAKMRFAHI 630 DGDHLTLLNV 640 YHAFKQNHES 650 VQWCYDNFIN 660 YRSLMSADNV 670 RQQLSRIMDR 680 FNLPRRSTDF 690 TSRDYYINIR 700 KALVTGYFMQ 710 VAHLERTGHY 720 LTVKDNQVVQ 730 LHPSTVLDHK 740 PEWVLYNEFV 750 LTTKNYIRTC 760 TDIKPEWLVK 770 IAPQYYDMSN 780 FPQCEAKRQL 790 DRIIAKLQSK EYSQY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0005689 U12-type spliceosomal complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0008186 ATP-dependent activity, acting on RNA
Molecular Function GO:0003725 double-stranded RNA binding
Molecular Function GO:0004386 helicase activity
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005730 nucleolus
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0140374 antiviral innate immune response
Biological Process GO:0042742 defense response to bacterium
Biological Process GO:0051607 defense response to virus
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0043123 positive regulation of canonical NF-kappaB signal transduction
Biological Process GO:0043279 response to alkaloid
Biological Process GO:0009636 response to toxic substance
Biological Process GO:0008380 RNA splicing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[7] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[8] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[9] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[10] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.