Overview
| Uniprot ID | O43151 |
| Protein Name | Methylcytosine dioxygenase TET3 |
| Gene Name | TET3 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 1012 |
RLAGDNPKEEEVLRK |
Function
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709). Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034)
Protein Sequence
10
MSQFQVPLAV
20
QPDLPGLYDF
30
PQRQVMVGSF
40
PGSGLSMAGS
50
ESQLRGGGDG
60
RKKRKRCGTC
70
EPCRRLENCG
80
ACTSCTNRRT
90
HQICKLRKCE
100
VLKKKVGLLK
110
EVEIKAGEGA
120
GPWGQGAAVK
130
TGSELSPVDG
140
PVPGQMDSGP
150
VYHGDSRQLS
160
ASGVPVNGAR
170
EPAGPSLLGT
180
GGPWRVDQKP
190
DWEAAPGPAH
200
TARLEDAHDL
210
VAFSAVAEAV
220
SSYGALSTRL
230
YETFNREMSR
240
EAGNNSRGPR
250
PGPEGCSAGS
260
EDLDTLQTAL
270
ALARHGMKPP
280
NCNCDGPECP
290
DYLEWLEGKI
300
KSVVMEGGEE
310
RPRLPGPLPP
320
GEAGLPAPST
330
RPLLSSEVPQ
340
ISPQEGLPLS
350
QSALSIAKEK
360
NISLQTAIAI
370
EALTQLSSAL
380
PQPSHSTPQA
390
SCPLPEALSP
400
PAPFRSPQSY
410
LRAPSWPVVP
420
PEEHSSFAPD
430
SSAFPPATPR
440
TEFPEAWGTD
450
TPPATPRSSW
460
PMPRPSPDPM
470
AELEQLLGSA
480
SDYIQSVFKR
490
PEALPTKPKV
500
KVEAPSSSPA
510
PAPSPVLQRE
520
APTPSSEPDT
530
HQKAQTALQQ
540
HLHHKRSLFL
550
EQVHDTSFPA
560
PSEPSAPGWW
570
PPPSSPVPRL
580
PDRPPKEKKK
590
KLPTPAGGPV
600
GTEKAAPGIK
610
PSVRKPIQIK
620
KSRPREAQPL
630
FPPVRQIVLE
640
GLRSPASQEV
650
QAHPPAPLPA
660
SQGSAVPLPP
670
EPSLALFAPS
680
PSRDSLLPPT
690
QEMRSPSPMT
700
ALQPGSTGPL
710
PPADDKLEEL
720
IRQFEAEFGD
730
SFGLPGPPSV
740
PIQDPENQQT
750
CLPAPESPFA
760
TRSPKQIKIE
770
SSGAVTVLST
780
TCFHSEEGGQ
790
EATPTKAENP
800
LTPTLSGFLE
810
SPLKYLDTPT
820
KSLLDTPAKR
830
AQAEFPTCDC
840
VEQIVEKDEG
850
PYYTHLGSGP
860
TVASIRELME
870
ERYGEKGKAI
880
RIEKVIYTGK
890
EGKSSRGCPI
900
AKWVIRRHTL
910
EEKLLCLVRH
920
RAGHHCQNAV
930
IVILILAWEG
940
IPRSLGDTLY
950
QELTDTLRKY
960
GNPTSRRCGL
970
NDDRTCACQG
980
KDPNTCGASF
990
SFGCSWSMYF
1000
NGCKYARSKT
1010
PRKFRLAGDN
1020
PKEEEVLRKS
1030
FQDLATEVAP
1040
LYKRLAPQAY
1050
QNQVTNEEIA
1060
IDCRLGLKEG
1070
RPFAGVTACM
1080
DFCAHAHKDQ
1090
HNLYNGCTVV
1100
CTLTKEDNRC
1110
VGKIPEDEQL
1120
HVLPLYKMAN
1130
TDEFGSEENQ
1140
NAKVGSGAIQ
1150
VLTAFPREVR
1160
RLPEPAKSCR
1170
QRQLEARKAA
1180
AEKKKIQKEK
1190
LSTPEKIKQE
1200
ALELAGITSD
1210
PGLSLKGGLS
1220
QQGLKPSLKV
1230
EPQNHFSSFK
1240
YSGNAVVESY
1250
SVLGNCRPSD
1260
PYSMNSVYSY
1270
HSYYAQPSLT
1280
SVNGFHSKYA
1290
LPSFSYYGFP
1300
SSNPVFPSQF
1310
LGPGAWGHSG
1320
SSGSFEKKPD
1330
LHALHNSLSP
1340
AYGGAEFAEL
1350
PSQAVPTDAH
1360
HPTPHHQQPA
1370
YPGPKEYLLP
1380
KAPLLHSVSR
1390
DPSPFAQSSN
1400
CYNRSIKQEP
1410
VDPLTQAEPV
1420
PRDAGKMGKT
1430
PLSEVSQNGG
1440
PSHLWGQYSG
1450
GPSMSPKRTN
1460
GVGGSWGVFS
1470
SGESPAIVPD
1480
KLSSFGASCL
1490
APSHFTDGQW
1500
GLFPGEGQQA
1510
ASHSGGRLRG
1520
KPWSPCKFGN
1530
STSALAGPSL
1540
TEKPWALGAG
1550
DFNSALKGSP
1560
GFQDKLWNPM
1570
KGEEGRIPAA
1580
GASQLDRAWQ
1590
SFGLPLGSSE
1600
KLFGALKSEE
1610
KLWDPFSLEE
1620
GPAEEPPSKG
1630
AVKEEKGGGG
1640
AEEEEEELWS
1650
DSEHNFLDEN
1660
IGGVAVAPAH
1670
GSILIECARR
1680
ELHATTPLKK
1690
PNRCHPTRIS
1700
LVFYQHKNLN
1710
QPNHGLALWE
1720
AKMKQLAERA
1730
RARQEEAARL
1740
GLGQQEAKLY
1750
GKKRKWGGTV
1760
VAEPQQKEKK
1770
GVVPTRQALA
1780
VPTDSAVTVS
1790
SYAYTKVTGP
YSRWI
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0001939 |
female pronucleus |
| Cellular Component |
GO:0001940 |
male pronucleus |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0070579 |
DNA 5-methylcytosine dioxygenase activity |
| Molecular Function |
GO:0008327 |
methyl-CpG binding |
| Molecular Function |
GO:0000978 |
RNA polymerase II cis-regulatory region sequence-specific DNA binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0044727 |
epigenetic programing of male pronucleus |
| Biological Process |
GO:0044029 |
positive regulation of gene expression via chromosomal CpG island demethylation |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0006493 |
protein O-linked glycosylation |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.