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Overview

Uniprot IDO43164
Protein NameE3 ubiquitin-protein ligase Praja-2
Gene NamePJA2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
320 VVRPKVRKLISSSQV

Function

Has E2-dependent E3 ubiquitin-protein ligase activity (PubMed:12036302, PubMed:21423175). Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes (PubMed:21423175). Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype (PubMed:28471450). Plays a role in ciliogenesis by ubiquitinating OFD1 (PubMed:33934390)

Protein Sequence

10 MSQYTEKEPA 20 AMDQESGKAV 30 WPKPAGGYQT 40 ITGRRYGRRH 50 AYVSFKPCMT 60 RHERSLGRAG 70 DDYEVLELDD 80 VPKENSSGSS 90 PLDQVDSSLP 100 SEPIFEKSET 110 EIPTCGSALN 120 QTTESSQSFV 130 AVHHSEEGRD 140 TLGSSTNLHN 150 HSEGEYIPGA 160 CSASSVQNGI 170 ALVHTDSYDP 180 DGKHGEDNDH 190 LQLSAEVVEG 200 SRYQESLGNT 210 VFELENREAE 220 AYTGLSPPVP 230 SFNCEVRDEF 240 EELDSVPLVK 250 SSAGDTEFVH 260 QNSQEIQRSS 270 QDEMVSTKQQ 280 NNTSQERQTE 290 HSPEDAACGP 300 GHICSEQNTN 310 DREKNHGSSP 320 EQVVRPKVRK 330 LISSSQVDQE 340 TGFNRHEAKQ 350 RSVQRWREAL 360 EVEESGSDDL 370 LIKCEEYDGE 380 HDCMFLDPPY 390 SRVITQRETE 400 NNQMTSESGA 410 TAGRQEVDNT 420 FWNGCGDYYQ 430 LYDKDEDSSE 440 CSDGEWSASL 450 PHRFSGTEKD 460 QSSSDESWET 470 LPGKDENEPE 480 LQSDSSGPEE 490 ENQELSLQEG 500 EQTSLEEGEI 510 PWLQYNEVNE 520 SSSDEGNEPA 530 NEFAQPAFML 540 DGNNNLEDDS 550 SVSEDLDVDW 560 SLFDGFADGL 570 GVAEAISYVD 580 PQFLTYMALE 590 ERLAQAMETA 600 LAHLESLAVD 610 VEVANPPASK 620 ESIDGLPETL 630 VLEDHTAIGQ 640 EQCCPICCSE 650 YIKDDIATEL 660 PCHHFFHKPC 670 VSIWLQKSGT 680 CPVCRRHFPP 690 AVIEASAAPS 700 SEPDPDAPPS NDSIAEAP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0034451 centriolar satellite
Cellular Component GO:0005813 centrosome
Cellular Component GO:0036064 ciliary basal body
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0098978 glutamatergic synapse
Cellular Component GO:0000139 Golgi membrane
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0014069 postsynaptic density
Molecular Function GO:0004862 cAMP-dependent protein kinase inhibitor activity
Molecular Function GO:0061630 ubiquitin protein ligase activity
Molecular Function GO:0004842 ubiquitin-protein transferase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006954 inflammatory response
Biological Process GO:0045087 innate immune response
Biological Process GO:0007616 long-term memory
Biological Process GO:0141163 positive regulation of cAMP/PKA signal transduction
Biological Process GO:0046330 positive regulation of JNK cascade
Biological Process GO:1900745 positive regulation of p38MAPK cascade
Biological Process GO:0034137 positive regulation of toll-like receptor 2 signaling pathway
Biological Process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0043030 regulation of macrophage activation

Reference

PMID: N/A