Search Results

Overview

Uniprot IDO43172
Protein NameU4/U6 small nuclear ribonucleoprotein Prp4
Gene NamePRPF4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
147 VGTDALKKTKKDDEK
15 STQATKTKAPDDLVA
26 DLVAPVVKKPHIYYG
27 LVAPVVKKPHIYYGS
45 KERERLAKGESGILG
53 GESGILGKDGLKAGI

Function

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

Protein Sequence

10 MASSRASSTQ 20 ATKTKAPDDL 30 VAPVVKKPHI 40 YYGSLEEKER 50 ERLAKGESGI 60 LGKDGLKAGI 70 EAGNINITSG 80 EVFEIEEHIS 90 ERQAEVLAEF 100 ERRKRARQIN 110 VSTDDSEVKA 120 CLRALGEPIT 130 LFGEGPAERR 140 ERLRNILSVV 150 GTDALKKTKK 160 DDEKSKKSKE 170 EYQQTWYHEG 180 PNSLKVARLW 190 IANYSLPRAM 200 KRLEEARLHK 210 EIPETTRTSQ 220 MQELHKSLRS 230 LNNFCSQIGD 240 DRPISYCHFS 250 PNSKMLATAC 260 WSGLCKLWSV 270 PDCNLLHTLR 280 GHNTNVGAIV 290 FHPKSTVSLD 300 PKDVNLASCA 310 ADGSVKLWSL 320 DSDEPVADIE 330 GHTVRVARVM 340 WHPSGRFLGT 350 TCYDRSWRLW 360 DLEAQEEILH 370 QEGHSMGVYD 380 IAFHQDGSLA 390 GTGGLDAFGR 400 VWDLRTGRCI 410 MFLEGHLKEI 420 YGINFSPNGY 430 HIATGSGDNT 440 CKVWDLRQRR 450 CVYTIPAHQN 460 LVTGVKFEPI 470 HGNFLLTGAY 480 DNTAKIWTHP 490 GWSPLKTLAG 500 HEGKVMGLDI 510 SSDGQLIATC 520 SYDRTFKLWM AE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0097525 spliceosomal snRNP complex
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0071001 U4/U6 snRNP
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex
Molecular Function GO:0030621 U4 snRNA binding
Molecular Function GO:0017070 U6 snRNA binding
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0006396 RNA processing
Biological Process GO:0008380 RNA splicing
Biological Process GO:0000375 RNA splicing, via transesterification reactions
Biological Process GO:0000245 spliceosomal complex assembly
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.