Search Results

Overview

Uniprot IDO43290
Protein NameU4/U6.U5 tri-snRNP-associated protein 1
Gene NameSART1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
170 QREELREKLAAAKEK
183 EKRLLNQKLGKIKTL
302 VDKERAEKNVELRKK
309 KNVELRKKKPDYLPY
336 KPRSILSKYDEELEG
657 LLETTVQKVARVKAP
666 ARVKAPNKSLPSAVY

Function

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA

Protein Sequence

10 MGSSKKHRGE 20 KEAAGTTAAA 30 GTGGATEQPP 40 RHREHKKHKH 50 RSGGSGGSGG 60 ERRKRSRERG 70 GERGSGRRGA 80 EAEARSSTHG 90 RERSQAEPSE 100 RRVKREKRDD 110 GYEAAASSKT 120 SSGDASSLSI 130 EETNKLRAKL 140 GLKPLEVNAI 150 KKEAGTKEEP 160 VTADVINPMA 170 LRQREELREK 180 LAAAKEKRLL 190 NQKLGKIKTL 200 GEDDPWLDDT 210 AAWIERSRQL 220 QKEKDLAEKR 230 AKLLEEMDQE 240 FGVSTLVEEE 250 FGQRRQDLYS 260 ARDLQGLTVE 270 HAIDSFREGE 280 TMILTLKDKG 290 VLQEEEDVLV 300 NVNLVDKERA 310 EKNVELRKKK 320 PDYLPYAEDE 330 SVDDLAQQKP 340 RSILSKYDEE 350 LEGERPHSFR 360 LEQGGTADGL 370 RERELEEIRA 380 KLRLQAQSLS 390 TVGPRLASEY 400 LTPEEMVTFK 410 KTKRRVKKIR 420 KKEKEVVVRA 430 DDLLPLGDQT 440 QDGDFGSRLR 450 GRGRRRVSEV 460 EEEKEPVPQP 470 LPSDDTRVEN 480 MDISDEEEGG 490 APPPGSPQVL 500 EEDEAELELQ 510 KQLEKGRRLR 520 QLQQLQQLRD 530 SGEKVVEIVK 540 KLESRQRGWE 550 EDEDPERKGA 560 IVFNATSEFC 570 RTLGEIPTYG 580 LAGNREEQEE 590 LMDFERDEER 600 SANGGSESDG 610 EENIGWSTVN 620 LDEEKQQQDF 630 SASSTTILDE 640 EPIVNRGLAA 650 ALLLCQNKGL 660 LETTVQKVAR 670 VKAPNKSLPS 680 AVYCIEDKMA 690 IDDKYSRREE 700 YRGFTQDFKE 710 KDGYKPDVKI 720 EYVDETGRKL 730 TPKEAFRQLS 740 HRFHGKGSGK 750 MKTERRMKKL 760 DEEALLKKMS 770 SSDTPLGTVA 780 LLQEKQKAQK 790 TPYIVLSGSG 800 KSMNANTITK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex
Molecular Function GO:0003723 RNA binding
Biological Process GO:0000481 maturation of 5S rRNA
Biological Process GO:0045292 mRNA cis splicing, via spliceosome
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0045585 positive regulation of cytotoxic T cell differentiation
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[6] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.