Search Results

Overview

Uniprot IDO43395
Protein NameU4/U6 small nuclear ribonucleoprotein Prp3
Gene NamePRPF3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
103 GDDSEISKESSGVKK
110 KESSGVKKRRIPRFE
139 ESPGMLTKLQIKQMM
282 THRMPTLKANIRAVK
328 QRQRRTFKFHDKGKF
337 HDKGKFEKIAQRLRT
376 RLALIAPKKELKEGD
377 LALIAPKKELKEGDI

Function

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

Protein Sequence

10 MALSKRELDE 20 LKPWIEKTVK 30 RVLGFSEPTV 40 VTAALNCVGK 50 GMDKKKAADH 60 LKPFLDDSTL 70 RFVDKLFEAV 80 EEGRSSRHSK 90 SSSDRSRKRE 100 LKEVFGDDSE 110 ISKESSGVKK 120 RRIPRFEEVE 130 EEPEVIPGPP 140 SESPGMLTKL 150 QIKQMMEAAT 160 RQIEERKKQL 170 SFISPPTPQP 180 KTPSSSQPER 190 LPIGNTIQPS 200 QAATFMNDAI 210 EKARKAAELQ 220 ARIQAQLALK 230 PGLIGNANMV 240 GLANLHAMGI 250 APPKVELKDQ 260 TKPTPLILDE 270 QGRTVDATGK 280 EIELTHRMPT 290 LKANIRAVKR 300 EQFKQQLKEK 310 PSEDMESNTF 320 FDPRVSIAPS 330 QRQRRTFKFH 340 DKGKFEKIAQ 350 RLRTKAQLEK 360 LQAEISQAAR 370 KTGIHTSTRL 380 ALIAPKKELK 390 EGDIPEIEWW 400 DSYIIPNGFD 410 LTEENPKRED 420 YFGITNLVEH 430 PAQLNPPVDN 440 DTPVTLGVYL 450 TKKEQKKLRR 460 QTRREAQKEL 470 QEKVRLGLMP 480 PPEPKVRISN 490 LMRVLGTEAV 500 QDPTKVEAHV 510 RAQMAKRQKA 520 HEEANAARKL 530 TAEQRKVKKI 540 KKLKEDISQG 550 VHISVYRVRN 560 LSNPAKKFKI 570 EANAGQLYLT 580 GVVVLHKDVN 590 VVVVEGGPKA 600 QKKFKRLMLH 610 RIKWDEQTSN 620 TKGDDDEESD 630 EEAVKKTNKC 640 VLVWEGTAKD 650 RSFGEMKFKQ 660 CPTENMAREH 670 FKKHGAEHYW 680 DLALSESVLE STD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0008380 RNA splicing
Biological Process GO:0000375 RNA splicing, via transesterification reactions
Biological Process GO:0000245 spliceosomal complex assembly
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.