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Overview

Uniprot IDO43633
Protein NameCharged multivesicular body protein 2a
Gene NameCHMP2A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
197 SVAAGGKKAEAAASA
94 SLKIQTLKSNNSMAQ

Function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 (PubMed:28242692). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4

Protein Sequence

10 MDLLFGRRKT 20 PEELLRQNQR 30 ALNRAMRELD 40 RERQKLETQE 50 KKIIADIKKM 60 AKQGQMDAVR 70 IMAKDLVRTR 80 RYVRKFVLMR 90 ANIQAVSLKI 100 QTLKSNNSMA 110 QAMKGVTKAM 120 GTMNRQLKLP 130 QIQKIMMEFE 140 RQAEIMDMKE 150 EMMNDAIDDA 160 MGDEEDEEES 170 DAVVSQVLDE 180 LGLSLTDELS 190 NLPSTGGSLS 200 VAAGGKKAEA 210 AASALADADA 220 DLEERLKNLR RD

Gene Ontology

Classification GO ID Description
Cellular Component GO:1904930 amphisome membrane
Cellular Component GO:0000421 autophagosome membrane
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000815 ESCRT III complex
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0005828 kinetochore microtubule
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0016020 membrane
Cellular Component GO:0030117 membrane coat
Cellular Component GO:0030496 midbody
Cellular Component GO:0005771 multivesicular body
Cellular Component GO:0032585 multivesicular body membrane
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0005643 nuclear pore
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0180020 membrane bending activity
Molecular Function GO:0031210 phosphatidylcholine binding
Molecular Function GO:0019904 protein domain specific binding
Biological Process GO:0097352 autophagosome maturation
Biological Process GO:0006914 autophagy
Biological Process GO:0032509 endosome transport via multivesicular body sorting pathway
Biological Process GO:1904903 ESCRT III complex disassembly
Biological Process GO:0045184 establishment of protein localization
Biological Process GO:0010458 exit from mitosis
Biological Process GO:1902774 late endosome to lysosome transport
Biological Process GO:0045324 late endosome to vacuole transport
Biological Process GO:0016236 macroautophagy
Biological Process GO:0090148 membrane fission
Biological Process GO:0010324 membrane invagination
Biological Process GO:0061952 midbody abscission
Biological Process GO:0007080 mitotic metaphase chromosome alignment
Biological Process GO:0036258 multivesicular body assembly
Biological Process GO:0071985 multivesicular body sorting pathway
Biological Process GO:0061763 multivesicular body-lysosome fusion
Biological Process GO:1903723 negative regulation of centriole elongation
Biological Process GO:0031468 nuclear membrane reassembly
Biological Process GO:0006997 nucleus organization
Biological Process GO:0001778 plasma membrane repair
Biological Process GO:1903543 positive regulation of exosomal secretion
Biological Process GO:0051260 protein homooligomerization
Biological Process GO:0051258 protein polymerization
Biological Process GO:0015031 protein transport
Biological Process GO:0010824 regulation of centrosome duplication
Biological Process GO:1901673 regulation of mitotic spindle assembly
Biological Process GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Biological Process GO:0051469 vesicle fusion with vacuole
Biological Process GO:0046761 viral budding from plasma membrane
Biological Process GO:0039702 viral budding via host ESCRT complex
Biological Process GO:0019076 viral release from host cell

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.