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Overview

Uniprot IDO43639
Protein NameCytoplasmic protein NCK2
Gene NameNCK2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
72 GSLVKNLKDTLGLGK
79 KDTLGLGKTRRKTSA

Function

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling

Protein Sequence

10 MTEEVIVIAK 20 WDYTAQQDQE 30 LDIKKNERLW 40 LLDDSKTWWR 50 VRNAANRTGY 60 VPSNYVERKN 70 SLKKGSLVKN 80 LKDTLGLGKT 90 RRKTSARDAS 100 PTPSTDAEYP 110 ANGSGADRIY 120 DLNIPAFVKF 130 AYVAEREDEL 140 SLVKGSRVTV 150 MEKCSDGWWR 160 GSYNGQIGWF 170 PSNYVLEEVD 180 EAAAESPSFL 190 SLRKGASLSN 200 GQGSRVLHVV 210 QTLYPFSSVT 220 EEELNFEKGE 230 TMEVIEKPEN 240 DPEWWKCKNA 250 RGQVGLVPKN 260 YVVVLSDGPA 270 LHPAHAPQIS 280 YTGPSSSGRF 290 AGREWYYGNV 300 TRHQAECALN 310 ERGVEGDFLI 320 RDSESSPSDF 330 SVSLKASGKN 340 KHFKVQLVDN 350 VYCIGQRRFH 360 TMDELVEHYK 370 KAPIFTSEHG 380 EKLYLVRALQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0014069 postsynaptic density
Cellular Component GO:0012506 vesicle membrane
Molecular Function GO:0008093 cytoskeletal anchor activity
Molecular Function GO:0001784 phosphotyrosine residue binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0030971 receptor tyrosine kinase binding
Molecular Function GO:0097110 scaffold protein binding
Molecular Function GO:0035591 signaling adaptor activity
Molecular Function GO:0030159 signaling receptor complex adaptor activity
Biological Process GO:0016477 cell migration
Biological Process GO:0048013 ephrin receptor signaling pathway
Biological Process GO:0007173 epidermal growth factor receptor signaling pathway
Biological Process GO:0008285 negative regulation of cell population proliferation
Biological Process GO:1903898 negative regulation of PERK-mediated unfolded protein response
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0030838 positive regulation of actin filament polymerization
Biological Process GO:1902237 positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
Biological Process GO:0042102 positive regulation of T cell proliferation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0036493 positive regulation of translation in response to endoplasmic reticulum stress
Biological Process GO:0036491 regulation of translation initiation in response to endoplasmic reticulum stress
Biological Process GO:0007172 signal complex assembly
Biological Process GO:0007165 signal transduction
Biological Process GO:0042110 T cell activation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.