Overview
| Uniprot ID | O43684 |
|---|
| Protein Name | Mitotic checkpoint protein BUB3 |
|---|
| Gene Name | BUB3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 216 |
DPSPEVQKKKYAFKC |
| 217 |
PSPEVQKKKYAFKCH |
Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1
Protein Sequence
10
MTGSNEFKLN
20
QPPEDGISSV
30
KFSPNTSQFL
40
LVSSWDTSVR
50
LYDVPANSMR
60
LKYQHTGAVL
70
DCAFYDPTHA
80
WSGGLDHQLK
90
MHDLNTDQEN
100
LVGTHDAPIR
110
CVEYCPEVNV
120
MVTGSWDQTV
130
KLWDPRTPCN
140
AGTFSQPEKV
150
YTLSVSGDRL
160
IVGTAGRRVL
170
VWDLRNMGYV
180
QQRRESSLKY
190
QTRCIRAFPN
200
KQGYVLSSIE
210
GRVAVEYLDP
220
SPEVQKKKYA
230
FKCHRLKENN
240
IEQIYPVNAI
250
SFHNIHNTFA
260
TGGSDGFVNI
270
WDPFNKKRLC
280
QFHRYPTSIA
290
SLAFSNDGTT
300
LAIASSYMYE
310
MDDTEHPEDG
320
IFIRQVTDAE
TKPKSPCT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:1990298 |
bub1-bub3 complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0000776 |
kinetochore |
| Cellular Component |
GO:0033597 |
mitotic checkpoint complex |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Biological Process |
GO:0008608 |
attachment of spindle microtubules to kinetochore |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0051321 |
meiotic cell cycle |
| Biological Process |
GO:0007094 |
mitotic spindle assembly checkpoint signaling |
| Biological Process |
GO:0034501 |
protein localization to kinetochore |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.