Search Results

Overview

Uniprot IDO43707
Protein NameAlpha-actinin-4
Gene NameACTN4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
114 HKINNVNKALDFIAS
122 ALDFIASKGVKLVSI
125 FIASKGVKLVSIGAE
181 QRKTAPYKNVNVQNF
214 PELIEYDKLRKDDPV
283 TAANRICKVLAVNQE
323 LEDRVPQKTIQEMQQ
331 TIQEMQQKLEDFRDY
417 RLDHLAEKFRQKASI
421 LAEKFRQKASIHEAW
432 HEAWTDGKEAMLKHR
437 DGKEAMLKHRDYETA
455 DIKALIRKHEAFESD
50 LLDPAWEKQQRKTFT
535 QLHLEYAKRAAPFNN
54 AWEKQQRKTFTAWCN
592 EAILAIHKEAQRIAE
604 IAESNHIKLSGSNPY
622 TPQIINSKWEKVQQL
625 IINSKWEKVQQLVPK
632 KVQQLVPKRDHALLE
66 WCNSHLRKAGTQIEN
760 QILTRDAKGISQEQM
779 ASFNHFDKDHGGALG
859 FKVLAGDKNFITAEE

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882)

Protein Sequence

10 MVDYHAANQS 20 YQYGPSSAGN 30 GAGGGGSMGD 40 YMAQEDDWDR 50 DLLLDPAWEK 60 QQRKTFTAWC 70 NSHLRKAGTQ 80 IENIDEDFRD 90 GLKLMLLLEV 100 ISGERLPKPE 110 RGKMRVHKIN 120 NVNKALDFIA 130 SKGVKLVSIG 140 AEEIVDGNAK 150 MTLGMIWTII 160 LRFAIQDISV 170 EETSAKEGLL 180 LWCQRKTAPY 190 KNVNVQNFHI 200 SWKDGLAFNA 210 LIHRHRPELI 220 EYDKLRKDDP 230 VTNLNNAFEV 240 AEKYLDIPKM 250 LDAEDIVNTA 260 RPDEKAIMTY 270 VSSFYHAFSG 280 AQKAETAANR 290 ICKVLAVNQE 300 NEHLMEDYEK 310 LASDLLEWIR 320 RTIPWLEDRV 330 PQKTIQEMQQ 340 KLEDFRDYRR 350 VHKPPKVQEK 360 CQLEINFNTL 370 QTKLRLSNRP 380 AFMPSEGKMV 390 SDINNGWQHL 400 EQAEKGYEEW 410 LLNEIRRLER 420 LDHLAEKFRQ 430 KASIHEAWTD 440 GKEAMLKHRD 450 YETATLSDIK 460 ALIRKHEAFE 470 SDLAAHQDRV 480 EQIAAIAQEL 490 NELDYYDSHN 500 VNTRCQKICD 510 QWDALGSLTH 520 SRREALEKTE 530 KQLEAIDQLH 540 LEYAKRAAPF 550 NNWMESAMED 560 LQDMFIVHTI 570 EEIEGLISAH 580 DQFKSTLPDA 590 DREREAILAI 600 HKEAQRIAES 610 NHIKLSGSNP 620 YTTVTPQIIN 630 SKWEKVQQLV 640 PKRDHALLEE 650 QSKQQSNEHL 660 RRQFASQANV 670 VGPWIQTKME 680 EIGRISIEMN 690 GTLEDQLSHL 700 KQYERSIVDY 710 KPNLDLLEQQ 720 HQLIQEALIF 730 DNKHTNYTME 740 HIRVGWEQLL 750 TTIARTINEV 760 ENQILTRDAK 770 GISQEQMQEF 780 RASFNHFDKD 790 HGGALGPEEF 800 KACLISLGYD 810 VENDRQGEAE 820 FNRIMSLVDP 830 NHSGLVTFQA 840 FIDFMSRETT 850 DTDTADQVIA 860 SFKVLAGDKN 870 FITAEELRRE 880 LPPDQAEYCI 890 ARMAPYQGPD 900 AVPGALDYKS 910 FSTALYGESD L

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0044325 transmembrane transporter binding
Biological Process GO:0030036 actin cytoskeleton organization
Biological Process GO:0055001 muscle cell development
Biological Process GO:1900025 negative regulation of substrate adhesion-dependent cell spreading
Biological Process GO:0035357 peroxisome proliferator activated receptor signaling pathway
Biological Process GO:0030335 positive regulation of cell migration
Biological Process GO:1901224 positive regulation of non-canonical NF-kappaB signal transduction
Biological Process GO:0032417 positive regulation of sodium:proton antiporter activity
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0015031 protein transport
Biological Process GO:0042981 regulation of apoptotic process
Biological Process GO:0048384 retinoic acid receptor signaling pathway
Biological Process GO:0033209 tumor necrosis factor-mediated signaling pathway
Biological Process GO:0030050 vesicle transport along actin filament
Cellular Component GO:0030054 cell junction
Cellular Component GO:0042995 cell projection
Cellular Component GO:0030864 cortical actin cytoskeleton
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0031093 platelet alpha granule lumen
Cellular Component GO:0098871 postsynaptic actin cytoskeleton
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0031143 pseudopodium
Cellular Component GO:1990904 ribonucleoprotein complex
Cellular Component GO:0001725 stress fiber
Cellular Component GO:0030018 Z disc
Molecular Function GO:0003779 actin binding
Molecular Function GO:0051015 actin filament binding
Molecular Function GO:0005509 calcium ion binding
Molecular Function GO:0031490 chromatin DNA binding
Molecular Function GO:0005178 integrin binding
Molecular Function GO:0016922 nuclear receptor binding
Molecular Function GO:0042974 nuclear retinoic acid receptor binding
Molecular Function GO:0001882 nucleoside binding

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.