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Overview

Uniprot IDO43776
Protein NameAsparagine--tRNA ligase, cytoplasmic
Gene NameNARS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
104 EAKKITIKNDPSLPE
38 KALMTVGKEPFPTIY
60 ERWNVISKSQLKNIK
64 VISKSQLKNIKKMWH
93 EDSLRREKNLEEAKK

Function

Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed:32738225, PubMed:32788587, PubMed:9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells (PubMed:12235211, PubMed:30171954). Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells (PubMed:32788587)

Protein Sequence

10 MVLAELYVSD 20 REGSDATGDG 30 TKEKPFKTGL 40 KALMTVGKEP 50 FPTIYVDSQK 60 ENERWNVISK 70 SQLKNIKKMW 80 HREQMKSESR 90 EKKEAEDSLR 100 REKNLEEAKK 110 ITIKNDPSLP 120 EPKCVKIGAL 130 EGYRGQRVKV 140 FGWVHRLRRQ 150 GKNLMFLVLR 160 DGTGYLQCVL 170 ADELCQCYNG 180 VLLSTESSVA 190 VYGMLNLTPK 200 GKQAPGGHEL 210 SCDFWELIGL 220 APAGGADNLI 230 NEESDVDVQL 240 NNRHMMIRGE 250 NMSKILKARS 260 MVTRCFRDHF 270 FDRGYYEVTP 280 PTLVQTQVEG 290 GATLFKLDYF 300 GEEAFLTQSS 310 QLYLETCLPA 320 LGDVFCIAQS 330 YRAEQSRTRR 340 HLAEYTHVEA 350 ECPFLTFDDL 360 LNRLEDLVCD 370 VVDRILKSPA 380 GSIVHELNPN 390 FQPPKRPFKR 400 MNYSDAIVWL 410 KEHDVKKEDG 420 TFYEFGEDIP 430 EAPERLMTDT 440 INEPILLCRF 450 PVEIKSFYMQ 460 RCPEDSRLTE 470 SVDVLMPNVG 480 EIVGGSMRIF 490 DSEEILAGYK 500 REGIDPTPYY 510 WYTDQRKYGT 520 CPHGGYGLGL 530 ERFLTWILNR 540 YHIRDVCLYP RFVQRCTP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Molecular Function GO:0004816 asparagine-tRNA ligase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0031728 CCR3 chemokine receptor binding
Molecular Function GO:0003676 nucleic acid binding
Molecular Function GO:0046983 protein dimerization activity
Biological Process GO:0006421 asparaginyl-tRNA aminoacylation
Biological Process GO:0016477 cell migration
Biological Process GO:0021987 cerebral cortex development
Biological Process GO:0006418 tRNA aminoacylation for protein translation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.