Search Results
Overview
| Uniprot ID | O43809 |
|---|---|
| Protein Name | Cleavage and polyadenylation specificity factor subunit 5 |
| Gene Name | NUDT21 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 23 | GVTQFGNKYIQQTKP |
| 29 | NKYIQQTKPLTLERT |
Function
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:14690600, PubMed:15937220, PubMed:17024186, PubMed:17098938, PubMed:29276085, PubMed:8626397, PubMed:9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:14690600, PubMed:17024186, PubMed:8626397, PubMed:9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:17098938, PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:17098938, PubMed:20695905, PubMed:29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed:29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed:14690600, PubMed:15169763, PubMed:17024186, PubMed:20479262, PubMed:22813749, PubMed:8626397). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed:20479262, PubMed:21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed:18445629)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005813 | centrosome |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005847 | mRNA cleavage and polyadenylation specificity factor complex |
| Cellular Component | GO:0005849 | mRNA cleavage factor complex |
| Cellular Component | GO:0016604 | nuclear body |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0042382 | paraspeckles |
| Molecular Function | GO:0003682 | chromatin binding |
| Molecular Function | GO:0042826 | histone deacetylase binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0035925 | mRNA 3'-UTR AU-rich region binding |
| Molecular Function | GO:0003729 | mRNA binding |
| Molecular Function | GO:0042803 | protein homodimerization activity |
| Molecular Function | GO:0003723 | RNA binding |
| Biological Process | GO:0030154 | cell differentiation |
| Biological Process | GO:0180010 | co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway |
| Biological Process | GO:0031124 | mRNA 3'-end processing |
| Biological Process | GO:0110104 | mRNA alternative polyadenylation |
| Biological Process | GO:0006397 | mRNA processing |
| Biological Process | GO:2000975 | positive regulation of pro-B cell differentiation |
| Biological Process | GO:2000738 | positive regulation of stem cell differentiation |
| Biological Process | GO:0010608 | post-transcriptional regulation of gene expression |
| Biological Process | GO:0051290 | protein heterotetramerization |
| Biological Process | GO:0051262 | protein tetramerization |
Reference
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