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Overview

Uniprot ID	O43823
Protein Name	A-kinase anchor protein 8
Gene Name	AKAP8
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
306	DGTGRKRKQFQLYEE

Function

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity)

Protein Sequence

10 MDQGYGGYGA 20 WSAGPANTQG 30 AYGTGVASWQ 40 GYENYNYYGA 50 QNTSVTTGAT 60 YSYGPASWEA 70 AKANDGGLAA 80 GAPAMHMASY 90 GPEPCTDNSD 100 SLIAKINQRL 110 DMMSKEGGRG 120 GSGGGGEGIQ 130 DRESSFRFQP 140 FESYDSRPCL 150 PEHNPYRPSY 160 SYDYEFDLGS 170 DRNGSFGGQY 180 SECRDPARER 190 GSLDGFMRGR 200 GQGRFQDRSN 210 PGTFMRSDPF 220 VPPAASSEPL 230 STPWNELNYV 240 GGRGLGGPSP 250 SRPPPSLFSQ 260 SMAPDYGVMG 270 MQGAGGYDST 280 MPYGCGRSQP 290 RMRDRDRPKR 300 RGFDRFGPDG 310 TGRKRKQFQL 320 YEEPDTKLAR 330 VDSEGDFSEN 340 DDAAGDFRSG 350 DEEFKGEDEL 360 CDSGRQRGEK 370 EDEDEDVKKR 380 REKQRRRDRT 390 RDRAADRIQF 400 ACSVCKFRSF 410 DDEEIQKHLQ 420 SKFHKETLRF 430 ISTKLPDKTV 440 EFLQEYIVNR 450 NKKIEKRRQE 460 LMEKETAKPK 470 PDPFKGIGQE 480 HFFKKIEAAH 490 CLACDMLIPA 500 QPQLLQRHLH 510 SVDHNHNRRL 520 AAEQFKKTSL 530 HVAKSVLNNR 540 HIVKMLEKYL 550 KGEDPFTSET 560 VDPEMEGDDN 570 LGGEDKKETP 580 EEVAADVLAE 590 VITAAVRAVD 600 GEGAPAPESS 610 GEPAEDEGPT 620 DTAEAGSDPQ 630 AEQLLEEQVP 640 CGTAHEKGVP 650 KARSEAAEAG 660 NGAETMAAEA 670 ESAQTRVAPA 680 PAAADAEVEQ 690 TDAESKDAVP TE

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0000793	condensed chromosome
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0001939	female pronucleus
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0016363	nuclear matrix
Cellular Component	GO:0005730	nucleolus
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0003690	double-stranded DNA binding
Molecular Function	GO:0042826	histone deacetylase binding
Molecular Function	GO:0051059	NF-kappaB binding
Molecular Function	GO:0034237	protein kinase A regulatory subunit binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0044839	cell cycle G2/M phase transition
Biological Process	GO:0071222	cellular response to lipopolysaccharide
Biological Process	GO:0071380	cellular response to prostaglandin E stimulus
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0045087	innate immune response
Biological Process	GO:0000278	mitotic cell cycle
Biological Process	GO:0007076	mitotic chromosome condensation
Biological Process	GO:0032720	negative regulation of tumor necrosis factor production
Biological Process	GO:0015031	protein transport
Biological Process	GO:0007165	signal transduction

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.