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Overview

Uniprot IDO55143
Protein NameSarcoplasmic/endoplasmic reticulum calcium ATPase 2
Gene NameAtp2a2
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
169 DIRLTSIKSTTLRVD
205 RAVNQDKKNMLFSGT
460 NVFDTELKGLSKIER
492 LEFSRDRKSMSVYCT
502 SVYCTPNKPSRTSMS
514 SMSKMFVKGAPEGVI
533 HIRVGSTKVPMTPGV
541 VPMTPGVKQKIMSVI
543 MTPGVKQKIMSVIRE
571 ATHDNPLKREEMHLE
712 NDAPALKKSEIGIAM

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes (By similarity). In coordination with FLVCR2 mediates heme-stimulated switching from mitochondrial ATP synthesis to thermogenesis

Protein Sequence

10 MENAHTKTVE 20 EVLGHFGVNE 30 STGLSLEQVK 40 KLKERWGSNE 50 LPAEEGKTLL 60 ELVIEQFEDL 70 LVRILLLAAC 80 ISFVLAWFEE 90 GEETITAFVE 100 PFVILLILVA 110 NAIVGVWQER 120 NAENAIEALK 130 EYEPEMGKVY 140 RQDRKSVQRI 150 KAKDIVPGDI 160 VEIAVGDKVP 170 ADIRLTSIKS 180 TTLRVDQSIL 190 TGESVSVIKH 200 TDPVPDPRAV 210 NQDKKNMLFS 220 GTNIAAGKAM 230 GVVVATGVNT 240 EIGKIRDEMV 250 ATEQERTPLQ 260 QKLDEFGEQL 270 SKVISLICIA 280 VWIINIGHFN 290 DPVHGGSWIR 300 GAIYYFKIAV 310 ALAVAAIPEG 320 LPAVITTCLA 330 LGTRRMAKKN 340 AIVRSLPSVE 350 TLGCTSVICS 360 DKTGTLTTNQ 370 MSVCRMFILD 380 KVEGDTCSLN 390 EFSITGSTYA 400 PIGEVQKDDK 410 PVKCHQYDGL 420 VELATICALC 430 NDSALDYNEA 440 KGVYEKVGEA 450 TETALTCLVE 460 KMNVFDTELK 470 GLSKIERANA 480 CNSVIKQLMK 490 KEFTLEFSRD 500 RKSMSVYCTP 510 NKPSRTSMSK 520 MFVKGAPEGV 530 IDRCTHIRVG 540 STKVPMTPGV 550 KQKIMSVIRE 560 WGSGSDTLRC 570 LALATHDNPL 580 KREEMHLEDS 590 ANFIKYETNL 600 TFVGCVGMLD 610 PPRIEVASSV 620 KLCRQAGIRV 630 IMITGDNKGT 640 AVAICRRIGI 650 FGQDEDVTSK 660 AFTGREFDEL 670 SPSAQRDACL 680 NARCFARVEP 690 SHKSKIVEFL 700 QSFDEITAMT 710 GDGVNDAPAL 720 KKSEIGIAMG 730 SGTAVAKTAS 740 EMVLADDNFS 750 TIVAAVEEGR 760 AIYNNMKQFI 770 RYLISSNVGE 780 VVCIFLTAAL 790 GFPEALIPVQ 800 LLWVNLVTDG 810 LPATALGFNP 820 PDLDIMNKPP 830 RNPKEPLISG 840 WLFFRYLAIG 850 CYVGAATVGA 860 AAWWFIAADG 870 GPRVSFYQLS 880 HFLQCKEDNP 890 DFDGVDCAIF 900 ESPYPMTMAL 910 SVLVTIEMCN 920 ALNSLSENQS 930 LLRMPPWENI 940 WLVGSICLSM 950 SLHFLILYVE 960 PLPLIFQITP 970 LNLTQWLMVL 980 KISLPVILMD 990 ETLKFVARNY 1000 LEQPGKECVQ 1010 PATKSSCSLS 1020 ACTDGISWPF 1030 VLLIMPLVVW 1040 VYSTDTNFSD MFWS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0061831 apical ectoplasmic specialization
Cellular Component GO:0090534 calcium ion-transporting ATPase complex
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0031234 extrinsic component of cytoplasmic side of plasma membrane
Cellular Component GO:0014801 longitudinal sarcoplasmic reticulum
Cellular Component GO:0016020 membrane
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0120025 plasma membrane bounded cell projection
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0097470 ribbon synapse
Cellular Component GO:0016529 sarcoplasmic reticulum
Cellular Component GO:0033017 sarcoplasmic reticulum membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0005246 calcium channel regulator activity
Molecular Function GO:0005509 calcium ion binding
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0106222 lncRNA binding
Molecular Function GO:0031775 lutropin-choriogonadotropic hormone receptor binding
Molecular Function GO:0005388 P-type calcium transporter activity
Molecular Function GO:0086039 P-type calcium transporter activity involved in regulation of cardiac muscle cell membrane potential
Molecular Function GO:0044548 S100 protein binding
Molecular Function GO:0044325 transmembrane transporter binding
Biological Process GO:0000045 autophagosome assembly
Biological Process GO:0016240 autophagosome membrane docking
Biological Process GO:1990036 calcium ion import into sarcoplasmic reticulum
Biological Process GO:0070588 calcium ion transmembrane transport
Biological Process GO:0006816 calcium ion transport
Biological Process GO:1903515 calcium ion transport from cytosol to endoplasmic reticulum
Biological Process GO:0014898 cardiac muscle hypertrophy in response to stress
Biological Process GO:0034599 cellular response to oxidative stress
Biological Process GO:0032469 endoplasmic reticulum calcium ion homeostasis
Biological Process GO:0006984 ER-nucleus signaling pathway
Biological Process GO:0006874 intracellular calcium ion homeostasis
Biological Process GO:1990456 mitochondrion-endoplasmic reticulum membrane tethering
Biological Process GO:0045822 negative regulation of heart contraction
Biological Process GO:0140056 organelle localization by membrane tethering
Biological Process GO:0010666 positive regulation of cardiac muscle cell apoptotic process
Biological Process GO:0032470 positive regulation of endoplasmic reticulum calcium ion concentration
Biological Process GO:1903233 regulation of calcium ion-dependent exocytosis of neurotransmitter
Biological Process GO:0098909 regulation of cardiac muscle cell action potential involved in regulation of contraction
Biological Process GO:0086036 regulation of cardiac muscle cell membrane potential
Biological Process GO:0010882 regulation of cardiac muscle contraction by calcium ion signaling
Biological Process GO:0006937 regulation of muscle contraction
Biological Process GO:0002026 regulation of the force of heart contraction
Biological Process GO:0055119 relaxation of cardiac muscle
Biological Process GO:0043434 response to peptide hormone
Biological Process GO:0070296 sarcoplasmic reticulum calcium ion transport
Biological Process GO:0033292 T-tubule organization
Biological Process GO:0014883 transition between fast and slow fiber

Reference

[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.