Search Results

Overview

Uniprot IDO60231
Protein NamePre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Gene NameDHX16
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1027 ELEDPHAKKMPKKIG
1028 LEDPHAKKMPKKIGK
131 KRKHLRKKREEEEEE
210 KAYEEAQKRLKMAEE
213 EEAQKRLKMAEEDRK
302 TNRYHMPKETRGQPA
340 RLGAASLKFGARDAA
73 LWNKVPRKAVVEKPA
78 PRKAVVEKPARAAER
93 EARALLEKNRSYRLL

Function

Required for pre-mRNA splicing as a component of the spliceosome (PubMed:20423332, PubMed:20841358, PubMed:25296192, PubMed:29360106). Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA (PubMed:35263596). Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses (PubMed:35263596)

Protein Sequence

10 MATPAGLERW 20 VQDELHSVLG 30 LSERHVAQFL 40 IGTAQRCTSA 50 EEFVQRLRDT 60 DTLDLSGPAR 70 DFALRLWNKV 80 PRKAVVEKPA 90 RAAEREARAL 100 LEKNRSYRLL 110 EDSEESSEET 120 VSRAGSSLQK 130 KRKKRKHLRK 140 KREEEEEEEA 150 SEKGKKKTGG 160 SKQQTEKPES 170 EDEWERTERE 180 RLQDLEERDA 190 FAERVRQRDK 200 DRTRNVLERS 210 DKKAYEEAQK 220 RLKMAEEDRK 230 AMVPELRKKS 240 RREYLAKRER 250 EKLEDLEAEL 260 ADEEFLFGDV 270 ELSRHERQEL 280 KYKRRVRDLA 290 REYRAAGEQE 300 KLEATNRYHM 310 PKETRGQPAR 320 AVDLVEEESG 330 APGEEQRRWE 340 EARLGAASLK 350 FGARDAASQE 360 PKYQLVLEEE 370 ETIEFVRATQ 380 LQGDEEPSAP 390 PTSTQAQQKE 400 SIQAVRRSLP 410 VFPFREELLA 420 AIANHQVLII 430 EGETGSGKTT 440 QIPQYLFEEG 450 YTNKGMKIAC 460 TQPRRVAAMS 470 VAARVAREMG 480 VKLGNEVGYS 490 IRFEDCTSER 500 TVLRYMTDGM 510 LLREFLSEPD 520 LASYSVVMVD 530 EAHERTLHTD 540 ILFGLIKDVA 550 RFRPELKVLV 560 ASATMDTARF 570 STFFDDAPVF 580 RIPGRRFPVD 590 IFYTKAPEAD 600 YLEACVVSVL 610 QIHVTQPPGD 620 ILVFLTGQEE 630 IEAACEMLQD 640 RCRRLGSKIR 650 ELLVLPIYAN 660 LPSDMQARIF 670 QPTPPGARKV 680 VVATNIAETS 690 LTIEGIIYVL 700 DPGFCKQKSY 710 NPRTGMESLT 720 VTPCSKASAN 730 QRAGRAGRVA 740 AGKCFRLYTA 750 WAYQHELEET 760 TVPEIQRTSL 770 GNVVLLLKSL 780 GIHDLMHFDF 790 LDPPPYETLL 800 LALEQLYALG 810 ALNHLGELTT 820 SGRKMAELPV 830 DPMLSKMILA 840 SEKYSCSEEI 850 LTVAAMLSVN 860 NSIFYRPKDK 870 VVHADNARVN 880 FFLPGGDHLV 890 LLNVYTQWAE 900 SGYSSQWCYE 910 NFVQFRSMRR 920 ARDVREQLEG 930 LLERVEVGLS 940 SCQGDYIRVR 950 KAITAGYFYH 960 TARLTRSGYR 970 TVKQQQTVFI 980 HPNSSLFEQQ 990 PRWLLYHELV 1000 LTTKEFMRQV 1010 LEIESSWLLE 1020 VAPHYYKAKE 1030 LEDPHAKKMP 1040 KKIGKTREEL G

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0004386 helicase activity
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0038187 pattern recognition receptor activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Molecular Function GO:0043130 ubiquitin binding
Biological Process GO:0140374 antiviral innate immune response
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0008380 RNA splicing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.