Search Results

Overview

Uniprot ID	O60313
Protein Name	Dynamin-like GTPase OPA1, mitochondrial
Gene Name	OPA1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
228	ELLHTQLKYQRILER
328	MMTRSPVKVTLSEGP
342	PHHVALFKDSSREFD

Function

Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain function (PubMed:16778770, PubMed:17709429, PubMed:20185555, PubMed:24616225, PubMed:28628083, PubMed:28746876, PubMed:31922487, PubMed:32228866, PubMed:32567732, PubMed:33130824, PubMed:33237841, PubMed:37612504, PubMed:37612506). Exists in two forms: the transmembrane, long form (Dynamin-like GTPase OPA1, long form; L-OPA1), which is tethered to the inner mitochondrial membrane, and the short soluble form (Dynamin-like GTPase OPA1, short form; S-OPA1), which results from proteolytic cleavage and localizes in the intermembrane space (PubMed:31922487, PubMed:32228866, PubMed:33237841, PubMed:37612504, PubMed:37612506). Both forms (L-OPA1 and S-OPA1) cooperate to catalyze the fusion of the mitochondrial inner membrane (PubMed:31922487, PubMed:37612504, PubMed:37612506). The equilibrium between L-OPA1 and S-OPA1 is essential: excess levels of S-OPA1, produced by cleavage by OMA1 following loss of mitochondrial membrane potential, lead to an impaired equilibrium between L-OPA1 and S-OPA1, inhibiting mitochondrial fusion (PubMed:20038677, PubMed:31922487). The balance between L-OPA1 and S-OPA1 also influences cristae shape and morphology (By similarity). Involved in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing by PARL in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Acts as a regulator of T-helper Th17 cells, which are characterized by cells with fused mitochondria with tight cristae, by mediating mitochondrial membrane remodeling: OPA1 is required for interleukin-17 (IL-17) production (By similarity). Its role in mitochondrial morphology is required for mitochondrial genome maintenance (PubMed:18158317, PubMed:20974897)

Protein Sequence

10 MWRLRRAAVA 20 CEVCQSLVKH 30 SSGIKGSLPL 40 QKLHLVSRSI 50 YHSHHPTLKL 60 QRPQLRTSFQ 70 QFSSLTNLPL 80 RKLKFSPIKY 90 GYQPRRNFWP 100 ARLATRLLKL 110 RYLILGSAVG 120 GGYTAKKTFD 130 QWKDMIPDLS 140 EYKWIVPDIV 150 WEIDEYIDFE 160 KIRKALPSSE 170 DLVKLAPDFD 180 KIVESLSLLK 190 DFFTSGSPEE 200 TAFRATDRGS 210 ESDKHFRKVS 220 DKEKIDQLQE 230 ELLHTQLKYQ 240 RILERLEKEN 250 KELRKLVLQK 260 DDKGIHHRKL 270 KKSLIDMYSE 280 VLDVLSDYDA 290 SYNTQDHLPR 300 VVVVGDQSAG 310 KTSVLEMIAQ 320 ARIFPRGSGE 330 MMTRSPVKVT 340 LSEGPHHVAL 350 FKDSSREFDL 360 TKEEDLAALR 370 HEIELRMRKN 380 VKEGCTVSPE 390 TISLNVKGPG 400 LQRMVLVDLP 410 GVINTVTSGM 420 APDTKETIFS 430 ISKAYMQNPN 440 AIILCIQDGS 450 VDAERSIVTD 460 LVSQMDPHGR 470 RTIFVLTKVD 480 LAEKNVASPS 490 RIQQIIEGKL 500 FPMKALGYFA 510 VVTGKGNSSE 520 SIEAIREYEE 530 EFFQNSKLLK 540 TSMLKAHQVT 550 TRNLSLAVSD 560 CFWKMVRESV 570 EQQADSFKAT 580 RFNLETEWKN 590 NYPRLRELDR 600 NELFEKAKNE 610 ILDEVISLSQ 620 VTPKHWEEIL 630 QQSLWERVST 640 HVIENIYLPA 650 AQTMNSGTFN 660 TTVDIKLKQW 670 TDKQLPNKAV 680 EVAWETLQEE 690 FSRFMTEPKG 700 KEHDDIFDKL 710 KEAVKEESIK 720 RHKWNDFAED 730 SLRVIQHNAL 740 EDRSISDKQQ 750 WDAAIYFMEE 760 ALQARLKDTE 770 NAIENMVGPD 780 WKKRWLYWKN 790 RTQEQCVHNE 800 TKNELEKMLK 810 CNEEHPAYLA 820 SDEITTVRKN 830 LESRGVEVDP 840 SLIKDTWHQV 850 YRRHFLKTAL 860 NHCNLCRRGF 870 YYYQRHFVDS 880 ELECNDVVLF 890 WRIQRMLAIT 900 ANTLRQQLTN 910 TEVRRLEKNV 920 KEVLEDFAED 930 GEKKIKLLTG 940 KRVQLAEDLK 950 KVREIQEKLD 960 AFIEALHQEK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:1904115	axon cytoplasm
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0030425	dendrite
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005874	microtubule
Cellular Component	GO:0030061	mitochondrial crista
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005758	mitochondrial intermembrane space
Cellular Component	GO:0031966	mitochondrial membrane
Biological Process	GO:0051259	protein complex oligomerization
Biological Process	GO:0007601	visual perception
Cellular Component	GO:0005741	mitochondrial outer membrane
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:1901612	cardiolipin binding
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0003924	GTPase activity
Molecular Function	GO:0140523	GTPase-dependent fusogenic activity
Molecular Function	GO:0000287	magnesium ion binding
Molecular Function	GO:0180020	membrane bending activity
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0070300	phosphatidic acid binding
Biological Process	GO:0006915	apoptotic process
Biological Process	GO:0019896	axonal transport of mitochondrion
Biological Process	GO:0090398	cellular senescence
Biological Process	GO:0042407	cristae formation
Biological Process	GO:0046039	GTP metabolic process
Biological Process	GO:0007007	inner mitochondrial membrane organization
Biological Process	GO:0097749	membrane tubulation
Biological Process	GO:0000266	mitochondrial fission
Biological Process	GO:0008053	mitochondrial fusion
Biological Process	GO:1990627	mitochondrial inner membrane fusion
Biological Process	GO:0007005	mitochondrion organization
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:1902236	negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
Biological Process	GO:0090201	negative regulation of release of cytochrome c from mitochondria
Biological Process	GO:0016559	peroxisome fission
Biological Process	GO:0032740	positive regulation of interleukin-17 production
Biological Process	GO:2000330	positive regulation of T-helper 17 cell lineage commitment

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.