Search Results

Overview

Uniprot IDO60341
Protein NameLysine-specific histone demethylase 1A
Gene NameKDM1A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
6 **MLSGKKAAAAAAA

Function

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:16079795, PubMed:16140033, PubMed:16223729, PubMed:27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:21300290, PubMed:26214369). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) (PubMed:15620353, PubMed:20389281, PubMed:21300290, PubMed:23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed:16079794, PubMed:16140033, PubMed:16885027, PubMed:21300290, PubMed:23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed:16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed:29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed:29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed:16079794, PubMed:16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed:20562920, PubMed:27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed:20389281). Required for the repression of GIPR expression (PubMed:34655521, PubMed:34906447)

Protein Sequence

10 MLSGKKAAAA 20 AAAAAAAATG 30 TEAGPGTAGG 40 SENGSEVAAQ 50 PAGLSGPAEV 60 GPGAVGERTP 70 RKKEPPRASP 80 PGGLAEPPGS 90 AGPQAGPTVV 100 PGSATPMETG 110 IAETPEGRRT 120 SRRKRAKVEY 130 REMDESLANL 140 SEDEYYSEEE 150 RNAKAEKEKK 160 LPPPPPQAPP 170 EEENESEPEE 180 PSGVEGAAFQ 190 SRLPHDRMTS 200 QEAACFPDII 210 SGPQQTQKVF 220 LFIRNRTLQL 230 WLDNPKIQLT 240 FEATLQQLEA 250 PYNSDTVLVH 260 RVHSYLERHG 270 LINFGIYKRI 280 KPLPTKKTGK 290 VIIIGSGVSG 300 LAAARQLQSF 310 GMDVTLLEAR 320 DRVGGRVATF 330 RKGNYVADLG 340 AMVVTGLGGN 350 PMAVVSKQVN 360 MELAKIKQKC 370 PLYEANGQAV 380 PKEKDEMVEQ 390 EFNRLLEATS 400 YLSHQLDFNV 410 LNNKPVSLGQ 420 ALEVVIQLQE 430 KHVKDEQIEH 440 WKKIVKTQEE 450 LKELLNKMVN 460 LKEKIKELHQ 470 QYKEASEVKP 480 PRDITAEFLV 490 KSKHRDLTAL 500 CKEYDELAET 510 QGKLEEKLQE 520 LEANPPSDVY 530 LSSRDRQILD 540 WHFANLEFAN 550 ATPLSTLSLK 560 HWDQDDDFEF 570 TGSHLTVRNG 580 YSCVPVALAE 590 GLDIKLNTAV 600 RQVRYTASGC 610 EVIAVNTRST 620 SQTFIYKCDA 630 VLCTLPLGVL 640 KQQPPAVQFV 650 PPLPEWKTSA 660 VQRMGFGNLN 670 KVVLCFDRVF 680 WDPSVNLFGH 690 VGSTTASRGE 700 LFLFWNLYKA 710 PILLALVAGE 720 AAGIMENISD 730 DVIVGRCLAI 740 LKGIFGSSAV 750 PQPKETVVSR 760 WRADPWARGS 770 YSYVAAGSSG 780 NDYDLMAQPI 790 TPGPSIPGAP 800 QPIPRLFFAG 810 EHTIRNYPAT 820 VHGALLSGLR 830 EAGRIADQFL 840 GAMYTLPRQA 850 TPGVPAQQSP SM

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0005667 transcription regulator complex
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0140297 DNA-binding transcription factor binding
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0140682 FAD-dependent H3K4me/H3K4me3 demethylase activity
Molecular Function GO:0050660 flavin adenine dinucleotide binding
Molecular Function GO:0032452 histone demethylase activity
Molecular Function GO:0032453 histone H3K4 demethylase activity
Molecular Function GO:0032454 histone H3K9 demethylase activity
Molecular Function GO:0140683 histone H3K9me/H3K9me2 demethylase activity
Molecular Function GO:0035575 histone H4K20 demethylase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0043426 MRF binding
Molecular Function GO:0050681 nuclear androgen receptor binding
Molecular Function GO:0016491 oxidoreductase activity
Molecular Function GO:0002039 p53 binding
Molecular Function GO:1990841 promoter-specific chromatin binding
Molecular Function GO:0140457 protein demethylase activity
Molecular Function GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
Molecular Function GO:0061752 telomeric repeat-containing RNA binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0003714 transcription corepressor activity
Biological Process GO:0071320 cellular response to cAMP
Biological Process GO:0071480 cellular response to gamma radiation
Biological Process GO:0034644 cellular response to UV
Biological Process GO:0021987 cerebral cortex development
Biological Process GO:0140861 DNA repair-dependent chromatin remodeling
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0040029 epigenetic regulation of gene expression
Biological Process GO:0046098 guanine metabolic process
Biological Process GO:0055001 muscle cell development
Biological Process GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:1902254 negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator
Biological Process GO:1902166 negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0160217 negative regulation of transcription initiation-coupled chromatin remodeling
Biological Process GO:0042551 neuron maturation
Biological Process GO:0048812 neuron projection morphogenesis
Biological Process GO:0120162 positive regulation of cold-induced thermogenesis
Biological Process GO:0010718 positive regulation of epithelial to mesenchymal transition
Biological Process GO:2000179 positive regulation of neural precursor cell proliferation
Biological Process GO:0002052 positive regulation of neuroblast proliferation
Biological Process GO:0031398 positive regulation of protein ubiquitination
Biological Process GO:2000648 positive regulation of stem cell proliferation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0060765 regulation of androgen receptor signaling pathway
Biological Process GO:0010569 regulation of double-strand break repair via homologous recombination
Biological Process GO:0032880 regulation of protein localization
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0060992 response to fungicide
Cellular Component GO:0000781 chromosome, telomeric region
Cellular Component GO:1990391 DNA repair complex
Cellular Component GO:0035097 histone methyltransferase complex

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.