Search Results

Overview

Uniprot IDO60488
Protein NameLong-chain-fatty-acid--CoA ligase 4
Gene NameACSL4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
117 PNGKVFKKLILGNYK
213 ELLESKLKTALLDIS
397 QEMNYIQKTLFKIGY
500 ICCEIKLKDWQEGGY
621 SFVVPNQKRLTLLAQ
661 REAANAMKLERFEIP
670 ERFEIPIKVRLSPEP
702 ELRNHYLKDIERMYG
89 LFDHAVSKFGKKDSL

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233, PubMed:31061331, PubMed:38720107). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET (PubMed:21242590). Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590). Acts as an activator of ferroptosis by activating polyunsaturated fatty acids, especially arachidonate and adrenate, to their active form, generating the primary lipid-peroxidation substrates that contribute to ferroptosis (PubMed:27842070, PubMed:35027735, PubMed:38720107)

Protein Sequence

10 MKLKLNVLTI 20 ILLPVHLLIT 30 IYSALIFIPW 40 YFLTNAKKKN 50 AMAKRIKAKP 60 TSDKPGSPYR 70 SVTHFDSLAV 80 IDIPGADTLD 90 KLFDHAVSKF 100 GKKDSLGTRE 110 ILSEENEMQP 120 NGKVFKKLIL 130 GNYKWMNYLE 140 VNRRVNNFGS 150 GLTALGLKPK 160 NTIAIFCETR 170 AEWMIAAQTC 180 FKYNFPLVTL 190 YATLGKEAVV 200 HGLNESEASY 210 LITSVELLES 220 KLKTALLDIS 230 CVKHIIYVDN 240 KAINKAEYPE 250 GFEIHSMQSV 260 EELGSNPENL 270 GIPPSRPTPS 280 DMAIVMYTSG 290 STGRPKGVMM 300 HHSNLIAGMT 310 GQCERIPGLG 320 PKDTYIGYLP 330 LAHVLELTAE 340 ISCFTYGCRI 350 GYSSPLTLSD 360 QSSKIKKGSK 370 GDCTVLKPTL 380 MAAVPEIMDR 390 IYKNVMSKVQ 400 EMNYIQKTLF 410 KIGYDYKLEQ 420 IKKGYDAPLC 430 NLLLFKKVKA 440 LLGGNVRMML 450 SGGAPLSPQT 460 HRFMNVCFCC 470 PIGQGYGLTE 480 SCGAGTVTEV 490 TDYTTGRVGA 500 PLICCEIKLK 510 DWQEGGYTIN 520 DKPNPRGEIV 530 IGGQNISMGY 540 FKNEEKTAED 550 YSVDENGQRW 560 FCTGDIGEFH 570 PDGCLQIIDR 580 KKDLVKLQAG 590 EYVSLGKVEA 600 ALKNCPLIDN 610 ICAFAKSDQS 620 YVISFVVPNQ 630 KRLTLLAQQK 640 GVEGTWVDIC 650 NNPAMEAEIL 660 KEIREAANAM 670 KLERFEIPIK 680 VRLSPEPWTP 690 ETGLVTDAFK 700 LKRKELRNHY 710 LKDIERMYGG K

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005811 lipid droplet
Cellular Component GO:0016020 membrane
Cellular Component GO:0044233 mitochondria-associated endoplasmic reticulum membrane contact site
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0047676 arachidonate-CoA ligase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004467 long-chain fatty acid-CoA ligase activity
Molecular Function GO:0090433 palmitoyl-CoA ligase activity
Molecular Function GO:0031957 very long-chain fatty acid-CoA ligase activity
Biological Process GO:0006633 fatty acid biosynthetic process
Biological Process GO:0006631 fatty acid metabolic process
Biological Process GO:0008610 lipid biosynthetic process
Biological Process GO:0006629 lipid metabolic process
Biological Process GO:0001676 long-chain fatty acid metabolic process
Biological Process GO:0035338 long-chain fatty-acyl-CoA biosynthetic process
Biological Process GO:0035336 long-chain fatty-acyl-CoA metabolic process
Biological Process GO:0032307 negative regulation of prostaglandin secretion
Biological Process GO:0030182 neuron differentiation
Biological Process GO:0030307 positive regulation of cell growth
Biological Process GO:0160020 positive regulation of ferroptosis
Biological Process GO:0032024 positive regulation of insulin secretion

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.