Search Results

Overview

Uniprot IDO60563
Protein NameCyclin-T1
Gene NameCCNT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
380 ISQKQNSKSVPSAKV
390 PSAKVSLKEYRAKHA
471 IPVAGGDKAASSKPE
481 SSKPEEIKMRIKVHA
540 LPVGTGNKRPGDPKH
590 AVFDHPAKIAKSTKS
593 DHPAKIAKSTKSSSL
638 KTRVPHSKLDKGPTG

Function

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II) (PubMed:16109376, PubMed:16109377, PubMed:30134174, PubMed:35393539). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II (PubMed:29849146, PubMed:35393539)

Protein Sequence

10 MEGERKNNNK 20 RWYFTREQLE 30 NSPSRRFGVD 40 PDKELSYRQQ 50 AANLLQDMGQ 60 RLNVSQLTIN 70 TAIVYMHRFY 80 MIQSFTQFPG 90 NSVAPAALFL 100 AAKVEEQPKK 110 LEHVIKVAHT 120 CLHPQESLPD 130 TRSEAYLQQV 140 QDLVILESII 150 LQTLGFELTI 160 DHPHTHVVKC 170 TQLVRASKDL 180 AQTSYFMATN 190 SLHLTTFSLQ 200 YTPPVVACVC 210 IHLACKWSNW 220 EIPVSTDGKH 230 WWEYVDATVT 240 LELLDELTHE 250 FLQILEKTPN 260 RLKRIWNWRA 270 CEAAKKTKAD 280 DRGTDEKTSE 290 QTILNMISQS 300 SSDTTIAGLM 310 SMSTSTTSAV 320 PSLPVSEESS 330 SNLTSVEMLP 340 GKRWLSSQPS 350 FKLEPTQGHR 360 TSENLALTGV 370 DHSLPQDGSN 380 AFISQKQNSK 390 SVPSAKVSLK 400 EYRAKHAEEL 410 AAQKRQLENM 420 EANVKSQYAY 430 AAQNLLSHHD 440 SHSSVILKMP 450 IEGSENPERP 460 FLEKADKTAL 470 KMRIPVAGGD 480 KAASSKPEEI 490 KMRIKVHAAA 500 DKHNSVEDSV 510 TKSREHKEKH 520 KTHPSNHHHH 530 HNHHSHKHSH 540 SQLPVGTGNK 550 RPGDPKHSSQ 560 TSNLAHKTYS 570 LSSSFSSSSS 580 TRKRGPSEET 590 GGAVFDHPAK 600 IAKSTKSSSL 610 NFSFPSLPTM 620 GQMPGHSSDT 630 SGLSFSQPSC 640 KTRVPHSKLD 650 KGPTGANGHN 660 TTQTIDYQDT 670 VNMLHSLLSA 680 QGVQPTQPTA 690 FEFVRPYSDY 700 LNPRSGGISS 710 RSGNTDKPRP 720 PPLPSEPPPP LPPLPK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0008024 cyclin/CDK positive transcription elongation factor complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0070691 P-TEFb complex
Molecular Function GO:0097322 7SK snRNA binding
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0061575 cyclin-dependent protein serine/threonine kinase activator activity
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0140297 DNA-binding transcription factor binding
Molecular Function GO:0140693 molecular condensate scaffold activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0070063 RNA polymerase binding
Molecular Function GO:0000976 transcription cis-regulatory region binding
Biological Process GO:0051301 cell division
Biological Process GO:0043923 host-mediated activation of viral transcription
Biological Process GO:0032786 positive regulation of DNA-templated transcription, elongation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0032968 positive regulation of transcription elongation by RNA polymerase II
Biological Process GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity
Biological Process GO:0006366 transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.