Overview
| Uniprot ID | O60566 |
|---|
| Protein Name | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta |
|---|
| Gene Name | BUB1B |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 231 |
RSTLAELKSKGKKTA |
| 233 |
TLAELKSKGKKTARA |
Function
Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle (PubMed:27509861). The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint (PubMed:11702782, PubMed:14706340, PubMed:15020684, PubMed:27509861). The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition (PubMed:10477750, PubMed:27509861). When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby reactivating APC/C for transition into anaphase (PubMed:27509861). MCC also monitors kinetochore activities that depend on the kinetochore motor CENPE (PubMed:15020684). Required for kinetochore localization of CENPE (PubMed:15020684). Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification (PubMed:19503101). May play a role for tumor suppression (By similarity)
Protein Sequence
10
MAAVKKEGGA
20
LSEAMSLEGD
30
EWELSKENVQ
40
PLRQGRIMST
50
LQGALAQESA
60
CNNTLQQQKR
70
AFEYEIRFYT
80
GNDPLDVWDR
90
YISWTEQNYP
100
QGGKESNMST
110
LLERAVEALQ
120
GEKRYYSDPR
130
FLNLWLKLGR
140
LCNEPLDMYS
150
YLHNQGIGVS
160
LAQFYISWAE
170
EYEARENFRK
180
ADAIFQEGIQ
190
QKAEPLERLQ
200
SQHRQFQARV
210
SRQTLLALEK
220
EEEEEVFESS
230
VPQRSTLAEL
240
KSKGKKTARA
250
PIIRVGGALK
260
APSQNRGLQN
270
PFPQQMQNNS
280
RITVFDENAD
290
EASTAELSKP
300
TVQPWIAPPM
310
PRAKENELQA
320
GPWNTGRSLE
330
HRPRGNTASL
340
IAVPAVLPSF
350
TPYVEETARQ
360
PVMTPCKIEP
370
SINHILSTRK
380
PGKEEGDPLQ
390
RVQSHQQASE
400
EKKEKMMYCK
410
EKIYAGVGEF
420
SFEEIRAEVF
430
RKKLKEQREA
440
ELLTSAEKRA
450
EMQKQIEEME
460
KKLKEIQTTQ
470
QERTGDQQEE
480
TMPTKETTKL
490
QIASESQKIP
500
GMTLSSSVCQ
510
VNCCARETSL
520
AENIWQEQPH
530
SKGPSVPFSI
540
FDEFLLSEKK
550
NKSPPADPPR
560
VLAQRRPLAV
570
LKTSESITSN
580
EDVSPDVCDE
590
FTGIEPLSED
600
AIITGFRNVT
610
ICPNPEDTCD
620
FARAARFVST
630
PFHEIMSLKD
640
LPSDPERLLP
650
EEDLDVKTSE
660
DQQTACGTIY
670
SQTLSIKKLS
680
PIIEDSREAT
690
HSSGFSGSSA
700
SVASTSSIKC
710
LQIPEKLELT
720
NETSENPTQS
730
PWCSQYRRQL
740
LKSLPELSAS
750
AELCIEDRPM
760
PKLEIEKEIE
770
LGNEDYCIKR
780
EYLICEDYKL
790
FWVAPRNSAE
800
LTVIKVSSQP
810
VPWDFYINLK
820
LKERLNEDFD
830
HFCSCYQYQD
840
GCIVWHQYIN
850
CFTLQDLLQH
860
SEYITHEITV
870
LIIYNLLTIV
880
EMLHKAEIVH
890
GDLSPRCLIL
900
RNRIHDPYDC
910
NKNNQALKIV
920
DFSYSVDLRV
930
QLDVFTLSGF
940
RTVQILEGQK
950
ILANCSSPYQ
960
VDLFGIADLA
970
HLLLFKEHLQ
980
VFWDGSFWKL
990
SQNISELKDG
1000
ELWNKFFVRI
1010
LNANDEATVS
1020
VLGELAAEMN
1030
GVFDTTFQSH
1040
LNKALWKVGK
1050
LTSPGALLFQ
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005680 |
anaphase-promoting complex |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0000776 |
kinetochore |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0000940 |
outer kinetochore |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005819 |
spindle |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0051754 |
meiotic sister chromatid cohesion, centromeric |
| Biological Process |
GO:0007094 |
mitotic spindle assembly checkpoint signaling |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.