Overview
| Uniprot ID | O60678 |
|---|
| Protein Name | Protein arginine N-methyltransferase 3 |
|---|
| Gene Name | PRMT3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 502 |
AGEALKGKVTVHKNK |
Function
Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (PubMed:22795084, PubMed:23445220, PubMed:25728001, PubMed:31378783, PubMed:33495566, PubMed:39513743). Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2 (PubMed:22795084). Also appears to methylate other ribosomal proteins (By similarity). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity (PubMed:33495566). Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation (PubMed:25728001, PubMed:31378783, PubMed:39513743). Mediates asymmetric arginine dimethylation of histone H4 'Arg-3' (H4R3me2a) in the promoter region of miRNA miR-3648, to promote its transcription and osteogenesis (PubMed:31378783)
Protein Sequence
10
MCSLASGATG
20
GRGAVENEED
30
LPELSDSGDE
40
AAWEDEDDAD
50
LPHGKQQTPC
60
LFCNRLFTSA
70
EETFSHCKSE
80
HQFNIDSMVH
90
KHGLEFYGYI
100
KLINFIRLKN
110
PTVEYMNSIY
120
NPVPWEKEEY
130
LKPVLEDDLL
140
LQFDVEDLYE
150
PVSVPFSYPN
160
GLSENTSVVE
170
KLKHMEARAL
180
SAEAALARAR
190
EDLQKMKQFA
200
QDFVMHTDVR
210
TCSSSTSVIA
220
DLQEDEDGVY
230
FSSYGHYGIH
240
EEMLKDKIRT
250
ESYRDFIYQN
260
PHIFKDKVVL
270
DVGCGTGILS
280
MFAAKAGAKK
290
VLGVDQSEIL
300
YQAMDIIRLN
310
KLEDTITLIK
320
GKIEEVHLPV
330
EKVDVIISEW
340
MGYFLLFESM
350
LDSVLYAKNK
360
YLAKGGSVYP
370
DICTISLVAV
380
SDVNKHADRI
390
AFWDDVYGFK
400
MSCMKKAVIP
410
EAVVEVLDPK
420
TLISEPCGIK
430
HIDCHTTSIS
440
DLEFSSDFTL
450
KITRTSMCTA
460
IAGYFDIYFE
470
KNCHNRVVFS
480
TGPQSTKTHW
490
KQTVFLLEKP
500
FSVKAGEALK
510
GKVTVHKNKK
520
DPRSLTVTLT
530
LNNSTQTYGL
Q
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0044020 |
histone H4R3 methyltransferase activity |
| Molecular Function |
GO:0042054 |
histone methyltransferase activity |
| Molecular Function |
GO:0008168 |
methyltransferase activity |
| Molecular Function |
GO:0016274 |
protein-arginine N-methyltransferase activity |
| Molecular Function |
GO:0035242 |
protein-arginine omega-N asymmetric methyltransferase activity |
| Molecular Function |
GO:0035241 |
protein-arginine omega-N monomethyltransferase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006338 |
chromatin remodeling |
| Biological Process |
GO:0031397 |
negative regulation of protein ubiquitination |
| Biological Process |
GO:1900053 |
negative regulation of retinoic acid biosynthetic process |
| Biological Process |
GO:0045669 |
positive regulation of osteoblast differentiation |
| Biological Process |
GO:0006479 |
protein methylation |
| Biological Process |
GO:0006355 |
regulation of DNA-templated transcription |
| Biological Process |
GO:0045815 |
transcription initiation-coupled chromatin remodeling |
Reference
[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.