Overview
| Uniprot ID | O60784 |
|---|
| Protein Name | Target of Myb1 membrane trafficking protein |
|---|
| Gene Name | TOM1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 106 |
LVRTILPKNNPPTIV |
Function
Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways (PubMed:14563850, PubMed:15047686, PubMed:23023224, PubMed:25588840, PubMed:26320582, PubMed:31371777). Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures (PubMed:23023224). Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes (PubMed:23023224). MYO6 links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN (PubMed:31371777). Binds to polyubiquitinated proteins via its GAT domain (PubMed:14563850). In a complex with TOLLIP, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed:15047686). The Tom1-Tollip complex may regulate endosomal trafficking by linking polyubiquitinated proteins to clathrin (PubMed:14563850, PubMed:15047686). Mediates clathrin recruitment to early endosomes by ZFYVE16 (PubMed:15657082). Modulates binding of TOLLIP to phosphatidylinositol 3-phosphate (PtdIns(3)P) via binding competition; the association with TOLLIP may favor the release of TOLLIP from endosomal membranes, allowing TOLLIP to commit to cargo trafficking (PubMed:26320582). Acts as a phosphatidylinositol 5-phosphate (PtdIns(5)P) effector by binding to PtdIns(5)P, thereby regulating endosomal maturation (PubMed:25588840). PtdIns(5)P-dependent recruitment to signaling endosomes may block endosomal maturation (PubMed:25588840). Also inhibits Toll-like receptor (TLR) signaling and participates in immune receptor recycling (PubMed:15047686, PubMed:26320582)
Protein Sequence
10
MDFLLGNPFS
20
SPVGQRIEKA
30
TDGSLQSEDW
40
ALNMEICDII
50
NETEEGPKDA
60
LRAVKKRIVG
70
NKNFHEVMLA
80
LTVLETCVKN
90
CGHRFHVLVA
100
SQDFVESVLV
110
RTILPKNNPP
120
TIVHDKVLNL
130
IQSWADAFRS
140
SPDLTGVVTI
150
YEDLRRKGLE
160
FPMTDLDMLS
170
PIHTPQRTVF
180
NSETQSGQDS
190
VGTDSSQQED
200
SGQHAAPLPA
210
PPILSGDTPI
220
APTPEQIGKL
230
RSELEMVSGN
240
VRVMSEMLTE
250
LVPTQAEPAD
260
LELLQELNRT
270
CRAMQQRVLE
280
LIPQIANEQL
290
TEELLIVNDN
300
LNNVFLRHER
310
FERFRTGQTT
320
KAPSEAEPAA
330
DLIDMGPDPA
340
ATGNLSSQLA
350
GMNLGSSSVR
360
AGLQSLEASG
370
RLEDEFDMFA
380
LTRGSSLADQ
390
RKEVKYEAPQ
400
ATDGLAGALD
410
ARQQSTGAIP
420
VTQACLMEDI
430
EQWLSTDVGN
440
DAEEPKGVTS
450
EEFDKFLEER
460
AKAADRLPNL
470
SSPSAEGPPG
480
PPSGPAPRKK
490
TQEKDDDMLF
AL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035577 |
azurophil granule membrane |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005769 |
early endosome |
| Cellular Component |
GO:0031901 |
early endosome membrane |
| Cellular Component |
GO:0005768 |
endosome |
| Cellular Component |
GO:0010008 |
endosome membrane |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0035579 |
specific granule membrane |
| Molecular Function |
GO:0030276 |
clathrin binding |
| Molecular Function |
GO:0032050 |
clathrin heavy chain binding |
| Molecular Function |
GO:0070853 |
myosin VI binding |
| Molecular Function |
GO:0010314 |
phosphatidylinositol-5-phosphate binding |
| Molecular Function |
GO:0031593 |
polyubiquitin modification-dependent protein binding |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Biological Process |
GO:0061909 |
autophagosome-lysosome fusion |
| Biological Process |
GO:0006897 |
endocytosis |
| Biological Process |
GO:0016197 |
endosomal transport |
| Biological Process |
GO:1901098 |
positive regulation of autophagosome maturation |
| Biological Process |
GO:0015031 |
protein transport |
| Biological Process |
GO:1904978 |
regulation of endosome organization |
| Biological Process |
GO:0007165 |
signal transduction |
| Biological Process |
GO:0061753 |
substrate localization to autophagosome |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.