Search Results

Overview

Uniprot IDO60869
Protein NameEndothelial differentiation-related factor 1
Gene NameEDF1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
131 IERAIGLKLRGKDIG
135 IGLKLRGKDIGKPIE
143 DIGKPIEKGPRAK**
15 TVTVLRKKGPTAAQA
23 GPTAAQAKSKQAILA
25 TAAQAKSKQAILAAQ
42 GEDVETSKKWAAGQN
43 EDVETSKKWAAGQNK
50 KWAAGQNKQHSITKN
56 NKQHSITKNTAKLDR
60 SITKNTAKLDRETEE

Function

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism

Protein Sequence

10 MAESDWDTVT 20 VLRKKGPTAA 30 QAKSKQAILA 40 AQRRGEDVET 50 SKKWAAGQNK 60 QHSITKNTAK 70 LDRETEELHH 80 DRVTLEVGKV 90 IQQGRQSKGL 100 TQKDLATKIN 110 EKPQVIADYE 120 SGRAIPNNQV 130 LGKIERAIGL 140 KLRGKDIGKP IEKGPRAK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005516 calmodulin binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0001094 TFIID-class transcription factor complex binding
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0045446 endothelial cell differentiation
Biological Process GO:0043388 positive regulation of DNA binding
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0019216 regulation of lipid metabolic process

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[6] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[7] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[8] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[9] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[10] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[11] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[12] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.