Search Results
Overview
| Uniprot ID | O75116 |
|---|---|
| Protein Name | Rho-associated protein kinase 2 |
| Gene Name | ROCK2 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 1031 | LLTERTLKTQAVNKL |
| 1368 | SSPRTSMKIQQNQSI |
| 720 | ARLADKNKIYESIEE |
| 946 | LEKEKIMKELEIKEM |
Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation
Protein Sequence
10
MSRPPPTGKM
20
PGAPETAPGD
30
GAGASRQRKL
40
EALIRDPRSP
50
INVESLLDGL
60
NSLVLDLDFP
70
ALRKNKNIDN
80
FLNRYEKIVK
90
KIRGLQMKAE
100
DYDVVKVIGR
110
GAFGEVQLVR
120
HKASQKVYAM
130
KLLSKFEMIK
140
RSDSAFFWEE
150
RDIMAFANSP
160
WVVQLFYAFQ
170
DDRYLYMVME
180
YMPGGDLVNL
190
MSNYDVPEKW
200
AKFYTAEVVL
210
ALDAIHSMGL
220
IHRDVKPDNM
230
LLDKHGHLKL
240
ADFGTCMKMD
250
ETGMVHCDTA
260
VGTPDYISPE
270
VLKSQGGDGF
280
YGRECDWWSV
290
GVFLYEMLVG
300
DTPFYADSLV
310
GTYSKIMDHK
320
NSLCFPEDAE
330
ISKHAKNLIC
340
AFLTDREVRL
350
GRNGVEEIRQ
360
HPFFKNDQWH
370
WDNIRETAAP
380
VVPELSSDID
390
SSNFDDIEDD
400
KGDVETFPIP
410
KAFVGNQLPF
420
IGFTYYRENL
430
LLSDSPSCRE
440
TDSIQSRKNE
450
ESQEIQKKLY
460
TLEEHLSNEM
470
QAKEELEQKC
480
KSVNTRLEKT
490
AKELEEEITL
500
RKSVESALRQ
510
LEREKALLQH
520
KNAEYQRKAD
530
HEADKKRNLE
540
NDVNSLKDQL
550
EDLKKRNQNS
560
QISTEKVNQL
570
QRQLDETNAL
580
LRTESDTAAR
590
LRKTQAESSK
600
QIQQLESNNR
610
DLQDKNCLLE
620
TAKLKLEKEF
630
INLQSALESE
640
RRDRTHGSEI
650
INDLQGRICG
660
LEEDLKNGKI
670
LLAKVELEKR
680
QLQERFTDLE
690
KEKSNMEIDM
700
TYQLKVIQQS
710
LEQEEAEHKA
720
TKARLADKNK
730
IYESIEEAKS
740
EAMKEMEKKL
750
LEERTLKQKV
760
ENLLLEAEKR
770
CSLLDCDLKQ
780
SQQKINELLK
790
QKDVLNEDVR
800
NLTLKIEQET
810
QKRCLTQNDL
820
KMQTQQVNTL
830
KMSEKQLKQE
840
NNHLMEMKMN
850
LEKQNAELRK
860
ERQDADGQMK
870
ELQDQLEAEQ
880
YFSTLYKTQV
890
RELKEECEEK
900
TKLGKELQQK
910
KQELQDERDS
920
LAAQLEITLT
930
KADSEQLARS
940
IAEEQYSDLE
950
KEKIMKELEI
960
KEMMARHKQE
970
LTEKDATIAS
980
LEETNRTLTS
990
DVANLANEKE
1000
ELNNKLKDVQ
1010
EQLSRLKDEE
1020
ISAAAIKAQF
1030
EKQLLTERTL
1040
KTQAVNKLAE
1050
IMNRKEPVKR
1060
GNDTDVRRKE
1070
KENRKLHMEL
1080
KSEREKLTQQ
1090
MIKYQKELNE
1100
MQAQIAEESQ
1110
IRIELQMTLD
1120
SKDSDIEQLR
1130
SQLQALHIGL
1140
DSSSIGSGPG
1150
DAEADDGFPE
1160
SRLEGWLSLP
1170
VRNNTKKFGW
1180
VKKYVIVSSK
1190
KILFYDSEQD
1200
KEQSNPYMVL
1210
DIDKLFHVRP
1220
VTQTDVYRAD
1230
AKEIPRIFQI
1240
LYANEGESKK
1250
EQEFPVEPVG
1260
EKSNYICHKG
1270
HEFIPTLYHF
1280
PTNCEACMKP
1290
LWHMFKPPPA
1300
LECRRCHIKC
1310
HKDHMDKKEE
1320
IIAPCKVYYD
1330
ISTAKNLLLL
1340
ANSTEEQQKW
1350
VSRLVKKIPK
1360
KPPAPDPFAR
1370
SSPRTSMKIQ
1380
QNQSIRRPSR
QLAPNKPS
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Biological Process | GO:0051893 | regulation of focal adhesion assembly |
| Biological Process | GO:0045616 | regulation of keratinocyte differentiation |
| Biological Process | GO:0031644 | regulation of nervous system process |
| Biological Process | GO:0051492 | regulation of stress fiber assembly |
| Biological Process | GO:1990776 | response to angiotensin |
| Biological Process | GO:0002931 | response to ischemia |
| Biological Process | GO:0071559 | response to transforming growth factor beta |
| Biological Process | GO:0007266 | Rho protein signal transduction |
| Biological Process | GO:0048511 | rhythmic process |
| Biological Process | GO:0006939 | smooth muscle contraction |
| Cellular Component | GO:0005813 | centrosome |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0036464 | cytoplasmic ribonucleoprotein granule |
| Cellular Component | GO:0005856 | cytoskeleton |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0005886 | plasma membrane |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0061133 | endopeptidase activator activity |
| Molecular Function | GO:0002020 | protease binding |
| Molecular Function | GO:0106310 | protein serine kinase activity |
| Molecular Function | GO:0004674 | protein serine/threonine kinase activity |
| Molecular Function | GO:0072518 | Rho-dependent protein serine/threonine kinase activity |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0031267 | small GTPase binding |
| Molecular Function | GO:0005198 | structural molecule activity |
| Molecular Function | GO:0048156 | tau protein binding |
| Molecular Function | GO:0050321 | tau-protein kinase activity |
| Molecular Function | GO:0008270 | zinc ion binding |
| Biological Process | GO:0030036 | actin cytoskeleton organization |
| Biological Process | GO:0031032 | actomyosin structure organization |
| Biological Process | GO:0003180 | aortic valve morphogenesis |
| Biological Process | GO:0097746 | blood vessel diameter maintenance |
| Biological Process | GO:1905145 | cellular response to acetylcholine |
| Biological Process | GO:0051298 | centrosome duplication |
| Biological Process | GO:0030866 | cortical actin cytoskeleton organization |
| Biological Process | GO:0048598 | embryonic morphogenesis |
| Biological Process | GO:0001837 | epithelial to mesenchymal transition |
| Biological Process | GO:0044788 | host-mediated perturbation of viral process |
| Biological Process | GO:0000281 | mitotic cytokinesis |
| Biological Process | GO:0061157 | mRNA destabilization |
| Biological Process | GO:0016525 | negative regulation of angiogenesis |
| Biological Process | GO:1903347 | negative regulation of bicellular tight junction assembly |
| Biological Process | GO:0070168 | negative regulation of biomineral tissue development |
| Biological Process | GO:0010629 | negative regulation of gene expression |
| Biological Process | GO:0045019 | negative regulation of nitric oxide biosynthetic process |
| Biological Process | GO:0150033 | negative regulation of protein localization to lysosome |
| Biological Process | GO:1902993 | positive regulation of amyloid precursor protein catabolic process |
| Biological Process | GO:1902004 | positive regulation of amyloid-beta formation |
| Biological Process | GO:0010613 | positive regulation of cardiac muscle hypertrophy |
| Biological Process | GO:0030335 | positive regulation of cell migration |
| Biological Process | GO:0010825 | positive regulation of centrosome duplication |
| Biological Process | GO:0032723 | positive regulation of connective tissue growth factor production |
| Biological Process | GO:1905205 | positive regulation of connective tissue replacement |
| Biological Process | GO:0090271 | positive regulation of fibroblast growth factor production |
| Biological Process | GO:0010628 | positive regulation of gene expression |
| Biological Process | GO:0043410 | positive regulation of MAPK cascade |
| Biological Process | GO:1902966 | positive regulation of protein localization to early endosome |
| Biological Process | GO:0051496 | positive regulation of stress fiber assembly |
| Biological Process | GO:0072659 | protein localization to plasma membrane |
| Biological Process | GO:0006468 | protein phosphorylation |
| Biological Process | GO:0032956 | regulation of actin cytoskeleton organization |
| Biological Process | GO:0110061 | regulation of angiotensin-activated signaling pathway |
| Biological Process | GO:0030155 | regulation of cell adhesion |
| Biological Process | GO:1901888 | regulation of cell junction assembly |
| Biological Process | GO:2000145 | regulation of cell motility |
| Biological Process | GO:1900037 | regulation of cellular response to hypoxia |
| Biological Process | GO:0042752 | regulation of circadian rhythm |
| Biological Process | GO:2000114 | regulation of establishment of cell polarity |
| Biological Process | GO:1903140 | regulation of establishment of endothelial barrier |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.
[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.