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Overview

Uniprot IDO75150
Protein NameE3 ubiquitin-protein ligase BRE1B
Gene NameRNF40
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
20 GGSGPPEKKLSREEK
420 RGLLLATKNSHLRHI
55 DLKVLQFKNKKLAER
57 KVLQFKNKKLAERLE
58 VLQFKNKKLAERLEQ

Function

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes

Protein Sequence

10 MSGPGNKRAA 20 GDGGSGPPEK 30 KLSREEKTTT 40 TLIEPIRLGG 50 ISSTEEMDLK 60 VLQFKNKKLA 70 ERLEQRQACE 80 DELRERIEKL 90 EKRQATDDAT 100 LLIVNRYWAQ 110 LDETVEALLR 120 CHESQGELSS 130 APEAPGTQEG 140 PTCDGTPLPE 150 PGTSELRDPL 160 LMQLRPPLSE 170 PALAFVVALG 180 ASSSEEVELE 190 LQGRMEFSKA 200 AVSRVVEASD 210 RLQRRVEELC 220 QRVYSRGDSE 230 PLSEAAQAHT 240 RELGRENRRL 250 QDLATQLQEK 260 HHRISLEYSE 270 LQDKVTSAET 280 KVLEMETTVE 290 DLQWDIEKLR 300 KREQKLNKHL 310 AEALEQLNSG 320 YYVSGSSSGF 330 QGGQITLSMQ 340 KFEMLNAELE 350 ENQELANSRM 360 AELEKLQAEL 370 QGAVRTNERL 380 KVALRSLPEE 390 VVRETGEYRM 400 LQAQFSLLYN 410 ESLQVKTQLD 420 EARGLLLATK 430 NSHLRHIEHM 440 ESDELGLQKK 450 LRTEVIQLED 460 TLAQVRKEYE 470 MLRIEFEQNL 480 AANEQAGPIN 490 REMRHLISSL 500 QNHNHQLKGD 510 AQRYKRKLRE 520 VQAEIGKLRA 530 QASGSAHSTP 540 NLGHPEDSGV 550 SAPAPGKEEG 560 GPGPVSTPDN 570 RKEMAPVPGT 580 TTTTTSVKKE 590 ELVPSEEDFQ 600 GITPGAQGPS 610 SRGREPEARP 620 KRELREREGP 630 SLGPPPVASA 640 LSRADREKAK 650 VEETKRKESE 660 LLKGLRAELK 670 KAQESQKEMK 680 LLLDMYKSAP 690 KEQRDKVQLM 700 AAERKAKAEV 710 DELRSRIREL 720 EERDRRESKK 730 IADEDALRRI 740 RQAEEQIEHL 750 QRKLGATKQE 760 EEALLSEMDV 770 TGQAFEDMQE 780 QNGRLLQQLR 790 EKDDANFKLM 800 SERIKANQIH 810 KLLREEKDEL 820 GEQVLGLKSQ 830 VDAQLLTVQK 840 LEEKERALQG 850 SLGGVEKELT 860 LRSQALELNK 870 RKAVEAAQLA 880 EDLKVQLEHV 890 QTRLREIQPC 900 LAESRAAREK 910 ESFNLKRAQE 920 DISRLRRKLE 930 KQRKVEVYAD 940 ADEILQEEIK 950 EYKARLTCPC 960 CNTRKKDAVL 970 TKCFHVFCFE 980 CVRGRYEARQ 990 RKCPKCNAAF 1000 GAHDFHRIYI S

Gene Ontology

Classification GO ID Description
Cellular Component GO:0033503 HULC complex
Cellular Component GO:0016020 membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000151 ubiquitin ligase complex
Molecular Function GO:0003730 mRNA 3'-UTR binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0061630 ubiquitin protein ligase activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006325 chromatin organization
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.