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Overview

Uniprot IDO75151
Protein NameLysine-specific demethylase PHF2
Gene NamePHF2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
266 YHVLKGEKTFYLIRP
569 PKKGKATKSVLSVPN
606 SEAKWKYKNSKPDSL
642 FSFSFSNKKLLGSKA
643 SFSFSNKKLLGSKAL

Function

Lysine demethylase that demethylates both histones and non-histone proteins (PubMed:20129925, PubMed:21167174, PubMed:21532585). Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B (PubMed:21532585). Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes (PubMed:21532585). The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA (PubMed:21532585). Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters (By similarity)

Protein Sequence

10 MATVPVYCVC 20 RLPYDVTRFM 30 IECDACKDWF 40 HGSCVGVEEE 50 EAPDIDIYHC 60 PNCEKTHGKS 70 TLKKKRTWHK 80 HGPGQAPDVK 90 PVQNGSQLFI 100 KELRSRTFPS 110 AEDVVARVPG 120 SQLTLGYMEE 130 HGFTEPILVP 140 KKDGLGLAVP 150 APTFYVSDVE 160 NYVGPERSVD 170 VTDVTKQKDC 180 KMKLKEFVDY 190 YYSTNRKRVL 200 NVTNLEFSDT 210 RMSSFVEPPD 220 IVKKLSWVEN 230 YWPDDALLAK 240 PKVTKYCLIC 250 VKDSYTDFHI 260 DSGGASAWYH 270 VLKGEKTFYL 280 IRPASANISL 290 YERWRSASNH 300 SEMFFADQVD 310 KCYKCIVKQG 320 QTLFIPSGWI 330 YATLTPVDCL 340 AFAGHFLHSL 350 SVEMQMRAYE 360 VERRLKLGSL 370 TQFPNFETAC 380 WYMGKHLLEA 390 FKGSHKSGKQ 400 LPPHLVQGAK 410 ILNGAFRSWT 420 KKQALAEHED 430 ELPEHFKPSQ 440 LIKDLAKEIR 450 LSENASKAVR 460 PEVNTVASSD 470 EVCDGDREKE 480 EPPSPIEATP 490 PQSLLEKVSK 500 KKTPKTVKMP 510 KPSKIPKPPK 520 PPKPPRPPKT 530 LKLKDGGKKK 540 GKKSRESASP 550 TIPNLDLLEA 560 HTKEALTKME 570 PPKKGKATKS 580 VLSVPNKDVV 590 HMQNDVERLE 600 IREQTKSKSE 610 AKWKYKNSKP 620 DSLLKMEEEQ 630 KLEKSPLAGN 640 KDNKFSFSFS 650 NKKLLGSKAL 660 RPPTSPGVFG 670 ALQNFKEDKP 680 KPVRDEYEYV 690 SDDGELKIDE 700 FPIRRKKNAP 710 KRDLSFLLDK 720 KAVLPTPVTK 730 PKLDSAAYKS 740 DDSSDEGSLH 750 IDTDTKPGRN 760 ARVKKESGSS 770 AAGILDLLQA 780 SEEVGALEYN 790 PSSQPPASPS 800 TQEAIQGMLS 810 MANLQASDSC 820 LQTTWGAGQA 830 KGSSLAAHGA 840 RKNGGGSGKS 850 AGKRLLKRAA 860 KNSVDLDDYE 870 EEQDHLDACF 880 KDSDYVYPSL 890 ESDEDNPIFK 900 SRSKKRKGSD 910 DAPYSPTARV 920 GPSVPRQDRP 930 VREGTRVASI 940 ETGLAAAAAK 950 LSQQEEQKSK 960 KKKSAKRKLT 970 PNTTSPSTST 980 SISAGTTSTS 990 TTPASTTPAS 1000 TTPASTSTAS 1010 SQASQEGSSP 1020 EPPPESHSSS 1030 LADHEYTAAG 1040 TFTGAQAGRT 1050 SQPMAPGVFL 1060 TQRRPSASSP 1070 NNNTAAKGKR 1080 TKKGMATAKQ 1090 RLGKILKIHR NGKLLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0032452 histone demethylase activity
Molecular Function GO:0140002 histone H3K4me3 reader activity
Molecular Function GO:0032454 histone H3K9 demethylase activity
Molecular Function GO:0035575 histone H4K20 demethylase activity
Molecular Function GO:0005506 iron ion binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0003712 transcription coregulator activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0001889 liver development
Biological Process GO:0061188 negative regulation of rDNA heterochromatin formation
Biological Process GO:0006482 protein demethylation
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.