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Overview

Uniprot IDO75351
Protein NameVacuolar protein sorting-associated protein 4B
Gene NameVPS4B
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
163 FPHLFTGKRTPWRGI

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission (PubMed:18687924). Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413)

Protein Sequence

10 MSSTSPNLQK 20 AIDLASKAAQ 30 EDKAGNYEEA 40 LQLYQHAVQY 50 FLHVVKYEAQ 60 GDKAKQSIRA 70 KCTEYLDRAE 80 KLKEYLKNKE 90 KKAQKPVKEG 100 QPSPADEKGN 110 DSDGEGESDD 120 PEKKKLQNQL 130 QGAIVIERPN 140 VKWSDVAGLE 150 GAKEALKEAV 160 ILPIKFPHLF 170 TGKRTPWRGI 180 LLFGPPGTGK 190 SYLAKAVATE 200 ANNSTFFSIS 210 SSDLVSKWLG 220 ESEKLVKNLF 230 QLARENKPSI 240 IFIDEIDSLC 250 GSRSENESEA 260 ARRIKTEFLV 270 QMQGVGVDND 280 GILVLGATNI 290 PWVLDSAIRR 300 RFEKRIYIPL 310 PEPHARAAMF 320 KLHLGTTQNS 330 LTEADFRELG 340 RKTDGYSGAD 350 ISIIVRDALM 360 QPVRKVQSAT 370 HFKKVRGPSR 380 ADPNHLVDDL 390 LTPCSPGDPG 400 AIEMTWMDVP 410 GDKLLEPVVS 420 MSDMLRSLSN 430 TKPTVNEHDL 440 LKLKKFTEDF GQEG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005768 endosome
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0090543 Flemming body
Cellular Component GO:0031902 late endosome membrane
Cellular Component GO:0030496 midbody
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0000922 spindle pole
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0097352 autophagosome maturation
Biological Process GO:0006914 autophagy
Biological Process GO:0016197 endosomal transport
Biological Process GO:0032510 endosome to lysosome transport via multivesicular body sorting pathway
Biological Process GO:1904903 ESCRT III complex disassembly
Biological Process GO:0061738 late endosomal microautophagy
Biological Process GO:0061764 late endosome to lysosome transport via multivesicular body sorting pathway
Biological Process GO:0016236 macroautophagy
Biological Process GO:0090148 membrane fission
Biological Process GO:0061952 midbody abscission
Biological Process GO:0007080 mitotic metaphase chromosome alignment
Biological Process GO:0036258 multivesicular body assembly
Biological Process GO:0071985 multivesicular body sorting pathway
Biological Process GO:1903542 negative regulation of exosomal secretion
Biological Process GO:0031468 nuclear membrane reassembly
Biological Process GO:0006997 nucleus organization
Biological Process GO:0001778 plasma membrane repair
Biological Process GO:1903724 positive regulation of centriole elongation
Biological Process GO:1903543 positive regulation of exosomal secretion
Biological Process GO:0010971 positive regulation of G2/M transition of mitotic cell cycle
Biological Process GO:0051261 protein depolymerization
Biological Process GO:0015031 protein transport
Biological Process GO:0010824 regulation of centrosome duplication
Biological Process GO:1901673 regulation of mitotic spindle assembly
Biological Process GO:0033993 response to lipid
Biological Process GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Biological Process GO:0090611 ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
Biological Process GO:0007033 vacuole organization
Biological Process GO:0046761 viral budding from plasma membrane
Biological Process GO:0039702 viral budding via host ESCRT complex

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.