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Overview

Uniprot IDO75356
Protein NameNucleoside diphosphate phosphatase ENTPD5
Gene NameENTPD5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
128 KKTPVVLKATAGLRL
142 LLPEHKAKALLFEVK
248 SYLGFGLKAARLATL
314 VLRVVRGKLHQPEEV
388 SYITALLKDGFGFAD

Function

Hydrolyzes nucleoside diphosphates with a preference for GDP, IDP and UDP compared to ADP and CDP (PubMed:10400613, PubMed:15698960). In the lumen of the endoplasmic reticulum, hydrolyzes UDP that acts as an end-product feedback inhibitor of the UDP-Glc:glycoprotein glucosyltransferases. UMP can be transported back by an UDP-sugar antiporter to the cytosol where it is consumed to regenerate UDP-glucose. Therefore, it positively regulates protein reglucosylation by clearing UDP from the ER lumen and by promoting the regeneration of UDP-glucose. Protein reglucosylation is essential to proper glycoprotein folding and quality control in the ER (By similarity)

Protein Sequence

10 MATSWGTVFF 20 MLVVSCVCSA 30 VSHRNQQTWF 40 EGIFLSSMCP 50 INVSASTLYG 60 IMFDAGSTGT 70 RIHVYTFVQK 80 MPGQLPILEG 90 EVFDSVKPGL 100 SAFVDQPKQG 110 AETVQGLLEV 120 AKDSIPRSHW 130 KKTPVVLKAT 140 AGLRLLPEHK 150 AKALLFEVKE 160 IFRKSPFLVP 170 KGSVSIMDGS 180 DEGILAWVTV 190 NFLTGQLHGH 200 RQETVGTLDL 210 GGASTQITFL 220 PQFEKTLEQT 230 PRGYLTSFEM 240 FNSTYKLYTH 250 SYLGFGLKAA 260 RLATLGALET 270 EGTDGHTFRS 280 ACLPRWLEAE 290 WIFGGVKYQY 300 GGNQEGEVGF 310 EPCYAEVLRV 320 VRGKLHQPEE 330 VQRGSFYAFS 340 YYYDRAVDTD 350 MIDYEKGGIL 360 KVEDFERKAR 370 EVCDNLENFT 380 SGSPFLCMDL 390 SYITALLKDG 400 FGFADSTVLQ 410 LTKKVNNIET 420 GWALGATFHL LQSLGISH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Molecular Function GO:0043262 ADP phosphatase activity
Molecular Function GO:0036384 CDP phosphatase activity
Molecular Function GO:0004382 GDP phosphatase activity
Molecular Function GO:1990003 IDP phosphatase activity
Molecular Function GO:0045134 UDP phosphatase activity
Biological Process GO:0051084 'de novo' post-translational protein folding
Biological Process GO:0006487 protein N-linked glycosylation
Biological Process GO:0006256 UDP catabolic process
Biological Process GO:0006011 UDP-alpha-D-glucose metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.