Search Results

Overview

Uniprot IDO75367
Protein NameCore histone macro-H2A.1
Gene NameMACROH2A1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
123 KKRGSKGKLEAIITP
134 IITPPPAKKAKSPSQ
135 ITPPPAKKAKSPSQK
137 PPPAKKAKSPSQKKP
142 KAKSPSQKKPVSKKA
161 GARKSKKKQGEVSKA
167 KKQGEVSKAASADST
301 CLALADDKKLKSIAF
302 LALADDKKLKSIAFP
304 LADDKKLKSIAFPSI

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription (PubMed:12718888, PubMed:15621527, PubMed:16428466). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (PubMed:15897469). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (PubMed:15897469). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation (PubMed:15897469). Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (PubMed:12718888, PubMed:16428466). Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (PubMed:16107708, PubMed:16428466)

Protein Sequence

10 MSSRGGKKKS 20 TKTSRSAKAG 30 VIFPVGRMLR 40 YIKKGHPKYR 50 IGVGAPVYMA 60 AVLEYLTAEI 70 LELAGNAARD 80 NKKGRVTPRH 90 ILLAVANDEE 100 LNQLLKGVTI 110 ASGGVLPNIH 120 PELLAKKRGS 130 KGKLEAIITP 140 PPAKKAKSPS 150 QKKPVSKKAG 160 GKKGARKSKK 170 KQGEVSKAAS 180 ADSTTEGTPA 190 DGFTVLSTKS 200 LFLGQKLQVV 210 QADIASIDSD 220 AVVHPTNTDF 230 YIGGEVGNTL 240 EKKGGKEFVE 250 AVLELRKKNG 260 PLEVAGAAVS 270 AGHGLPAKFV 280 IHCNSPVWGA 290 DKCEELLEKT 300 VKNCLALADD 310 KKLKSIAFPS 320 IGSGRNGFPK 330 QTAAQLILKA 340 ISSYFVSTMS 350 SSIKTVYFVL 360 FDSESIGIYV QEMAKLDAN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0001740 Barr body
Cellular Component GO:0000785 chromatin
Cellular Component GO:0000781 chromosome, telomeric region
Cellular Component GO:0000793 condensed chromosome
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0000228 nuclear chromosome
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000786 nucleosome
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005721 pericentric heterochromatin
Cellular Component GO:0001739 sex chromatin
Cellular Component GO:0090734 site of DNA damage
Molecular Function GO:0072570 ADP-D-ribose binding
Molecular Function GO:0160002 ADP-D-ribose modification-dependent protein binding
Molecular Function GO:0031490 chromatin DNA binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0010385 double-stranded methylated DNA binding
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0031492 nucleosomal DNA binding
Molecular Function GO:0160004 poly-ADP-D-ribose modification-dependent protein binding
Molecular Function GO:1990841 promoter-specific chromatin binding
Molecular Function GO:0046982 protein heterodimerization activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0030291 protein serine/threonine kinase inhibitor activity
Molecular Function GO:0000182 rDNA binding
Molecular Function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular Function GO:0030527 structural constituent of chromatin
Molecular Function GO:0000976 transcription cis-regulatory region binding
Biological Process GO:0006281 DNA repair
Biological Process GO:0009048 dosage compensation by inactivation of X chromosome
Biological Process GO:0040029 epigenetic regulation of gene expression
Biological Process GO:0071169 establishment of protein localization to chromatin
Biological Process GO:0031507 heterochromatin formation
Biological Process GO:1902750 negative regulation of cell cycle G2/M phase transition
Biological Process GO:0045814 negative regulation of gene expression, epigenetic
Biological Process GO:1904815 negative regulation of protein localization to chromosome, telomeric region
Biological Process GO:1902883 negative regulation of response to oxidative stress
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:1901837 negative regulation of transcription of nucleolar large rRNA by RNA polymerase I
Biological Process GO:0006334 nucleosome assembly
Biological Process GO:1903226 positive regulation of endodermal cell differentiation
Biological Process GO:0045618 positive regulation of keratinocyte differentiation
Biological Process GO:0034184 positive regulation of maintenance of mitotic sister chromatid cohesion
Biological Process GO:1902884 positive regulation of response to oxidative stress
Biological Process GO:0019216 regulation of lipid metabolic process
Biological Process GO:1902688 regulation of NAD metabolic process
Biological Process GO:0002082 regulation of oxidative phosphorylation
Biological Process GO:1902882 regulation of response to oxidative stress
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[6] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[7] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[8] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.