Overview
| Uniprot ID | O75525 |
|---|
| Protein Name | KH domain-containing, RNA-binding, signal transduction-associated protein 3 |
|---|
| Gene Name | KHDRBS3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 152 |
GHALEEIKKFLIPDY |
| 335 |
APSARTAKGVYRDQP |
Function
RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6 (PubMed:10564820, PubMed:19561594, PubMed:26758068). Involved in splice site selection of vascular endothelial growth factor (PubMed:15901763). In vitro regulates CD44 alternative splicing by direct binding to purine-rich exonic enhancer (By similarity). Can regulate alternative splicing of neurexins NRXN1-3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners such as neuroligins and LRRTM family members (PubMed:26758068). Targeted, cell-type specific splicing regulation of NRXN1 at AS4 is involved in neuronal glutamatergic synapse function and plasticity (By similarity). May regulate expression of KHDRBS2/SLIM-1 in defined brain neuron populations by modifying its alternative splicing (By similarity). Can bind FABP9 mRNA (By similarity). May play a role as a negative regulator of cell growth. Inhibits cell proliferation
Protein Sequence
10
MEEKYLPELM
20
AEKDSLDPSF
30
THALRLVNQE
40
IEKFQKGEGK
50
DEEKYIDVVI
60
NKNMKLGQKV
70
LIPVKQFPKF
80
NFVGKLLGPR
90
GNSLKRLQEE
100
TLTKMSILGK
110
GSMRDKAKEE
120
ELRKSGEAKY
130
FHLNDDLHVL
140
IEVFAPPAEA
150
YARMGHALEE
160
IKKFLIPDYN
170
DEIRQAQLQE
180
LTYLNGGSEN
190
ADVPVVRGKP
200
TLRTRGVPAP
210
AITRGRGGVT
220
ARPVGVVVPR
230
GTPTPRGVLS
240
TRGPVSRGRG
250
LLTPRARGVP
260
PTGYRPPPPP
270
PTQETYGEYD
280
YDDGYGTAYD
290
EQSYDSYDNS
300
YSTPAQSGAD
310
YYDYGHGLSE
320
ETYDSYGQEE
330
WTNSRHKAPS
340
ARTAKGVYRD
QPYGRY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0019904 |
protein domain specific binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0017124 |
SH3 domain binding |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
Reference
[1] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.