Overview
| Uniprot ID | O75569 |
|---|
| Protein Name | Interferon-inducible double-stranded RNA-dependent protein kinase activator A |
|---|
| Gene Name | PRKRA |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 225 |
LRNSPGEKINLLKRS |
| 230 |
GEKINLLKRSLLSIP |
Function
Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity). May function as regulator of gastric epithelial differentiation (By similarity)
Protein Sequence
10
MSQSRHRAEA
20
PPLEREDSGT
30
FSLGKMITAK
40
PGKTPIQVLH
50
EYGMKTKNIP
60
VYECERSDVQ
70
IHVPTFTFRV
80
TVGDITCTGE
90
GTSKKLAKHR
100
AAEAAINILK
110
ANASICFAVP
120
DPLMPDPSKQ
130
PKNQLNPIGS
140
LQELAIHHGW
150
RLPEYTLSQE
160
GGPAHKREYT
170
TICRLESFME
180
TGKGASKKQA
190
KRNAAEKFLA
200
KFSNISPENH
210
ISLTNVVGHS
220
LGCTWHSLRN
230
SPGEKINLLK
240
RSLLSIPNTD
250
YIQLLSEIAK
260
EQGFNITYLD
270
IDELSANGQY
280
QCLAELSTSP
290
ITVCHGSGIS
300
CGNAQSDAAH
310
NALQYLKIIA
ERK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0016442 |
RISC complex |
| Cellular Component |
GO:0070578 |
RISC-loading complex |
| Molecular Function |
GO:0003725 |
double-stranded RNA binding |
| Molecular Function |
GO:0008047 |
enzyme activator activity |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0070883 |
pre-miRNA binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0035197 |
siRNA binding |
| Biological Process |
GO:0140374 |
antiviral innate immune response |
| Biological Process |
GO:0006955 |
immune response |
| Biological Process |
GO:0035196 |
miRNA processing |
| Biological Process |
GO:0008285 |
negative regulation of cell population proliferation |
| Biological Process |
GO:0031054 |
pre-miRNA processing |
| Biological Process |
GO:0050821 |
protein stabilization |
| Biological Process |
GO:0070920 |
regulation of regulatory ncRNA processing |
| Biological Process |
GO:0009615 |
response to virus |
| Biological Process |
GO:0070922 |
RISC complex assembly |
| Biological Process |
GO:0030422 |
siRNA processing |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.