Overview
| Uniprot ID | O75608 |
|---|
| Protein Name | Acyl-protein thioesterase 1 |
|---|
| Gene Name | LYPLA1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 105 |
ALIDQEVKNGIPSNR |
| 190 |
SLTVEKLKTLVNPAN |
Function
Acts as an acyl-protein thioesterase (PubMed:19439193, PubMed:20418879). Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (PubMed:20418879). Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1 (PubMed:22399288, PubMed:27481942, PubMed:37802024). Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes (PubMed:37802024). Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (PubMed:19439193). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (By similarity). Has much higher thioesterase activity than lysophospholipase activity (PubMed:19439193). Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (PubMed:21393252)
Protein Sequence
10
MCGNNMSTPL
20
PAIVPAARKA
30
TAAVIFLHGL
40
GDTGHGWAEA
50
FAGIRSSHIK
60
YICPHAPVRP
70
VTLNMNVAMP
80
SWFDIIGLSP
90
DSQEDESGIK
100
QAAENIKALI
110
DQEVKNGIPS
120
NRIILGGFSQ
130
GGALSLYTAL
140
TTQQKLAGVT
150
ALSCWLPLRA
160
SFPQGPIGGA
170
NRDISILQCH
180
GDCDPLVPLM
190
FGSLTVEKLK
200
TLVNPANVTF
210
KTYEGMMHSS
220
CQQEMMDVKQ
230
FIDKLLPPID
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0031965 |
nuclear membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0004620 |
glycerophospholipase activity |
| Molecular Function |
GO:0016298 |
lipase activity |
| Molecular Function |
GO:0008474 |
palmitoyl-(protein) hydrolase activity |
| Molecular Function |
GO:0004622 |
phosphatidylcholine lysophospholipase A1 activity |
| Biological Process |
GO:0006631 |
fatty acid metabolic process |
| Biological Process |
GO:0015908 |
fatty acid transport |
| Biological Process |
GO:1905336 |
negative regulation of aggrephagy |
| Biological Process |
GO:0042997 |
negative regulation of Golgi to plasma membrane protein transport |
| Biological Process |
GO:0002084 |
protein depalmitoylation |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.