Search Results

Overview

Uniprot ID	O75643
Protein Name	U5 small nuclear ribonucleoprotein 200 kDa helicase
Gene Name	SNRNP200
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
46	EVLSLVGKLEGTRMG
83	EDRHDINKMKGYTLL
85	RHDINKMKGYTLLSE

Function

Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome (PubMed:35241646). Plays a role in pre-mRNA splicing as a core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:28502770, PubMed:28781166, PubMed:29301961, PubMed:29360106, PubMed:29361316, PubMed:30315277, PubMed:30705154, PubMed:30728453). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome

Protein Sequence

10 MADVTARSLQ 20 YEYKANSNLV 30 LQADRSLIDR 40 TRRDEPTGEV 50 LSLVGKLEGT 60 RMGDKAQRTK 70 PQMQEERRAK 80 RRKRDEDRHD 90 INKMKGYTLL 100 SEGIDEMVGI 110 IYKPKTKETR 120 ETYEVLLSFI 130 QAALGDQPRD 140 ILCGAADEVL 150 AVLKNEKLRD 160 KERRKEIDLL 170 LGQTDDTRYH 180 VLVNLGKKIT 190 DYGGDKEIQN 200 MDDNIDETYG 210 VNVQFESDEE 220 EGDEDVYGEV 230 REEASDDDME 240 GDEAVVRCTL 250 SANLVASGEL 260 MSSKKKDLHP 270 RDIDAFWLQR 280 QLSRFYDDAI 290 VSQKKADEVL 300 EILKTASDDR 310 ECENQLVLLL 320 GFNTFDFIKV 330 LRQHRMMILY 340 CTLLASAQSE 350 AEKERIMGKM 360 EADPELSKFL 370 YQLHETEKED 380 LIREERSRRE 390 RVRQSRMDTD 400 LETMDLDQGG 410 EALAPRQVLD 420 LEDLVFTQGS 430 HFMANKRCQL 440 PDGSFRRQRK 450 GYEEVHVPAL 460 KPKPFGSEEQ 470 LLPVEKLPKY 480 AQAGFEGFKT 490 LNRIQSKLYR 500 AALETDENLL 510 LCAPTGAGKT 520 NVALMCMLRE 530 IGKHINMDGT 540 INVDDFKIIY 550 IAPMRSLVQE 560 MVGSFGKRLA 570 TYGITVAELT 580 GDHQLCKEEI 590 SATQIIVCTP 600 EKWDIITRKG 610 GERTYTQLVR 620 LIILDEIHLL 630 HDDRGPVLEA 640 LVARAIRNIE 650 MTQEDVRLIG 660 LSATLPNYED 670 VATFLRVDPA 680 KGLFYFDNSF 690 RPVPLEQTYV 700 GITEKKAIKR 710 FQIMNEIVYE 720 KIMEHAGKNQ 730 VLVFVHSRKE 740 TGKTARAIRD 750 MCLEKDTLGL 760 FLREGSASTE 770 VLRTEAEQCK 780 NLELKDLLPY 790 GFAIHHAGMT 800 RVDRTLVEDL 810 FADKHIQVLV 820 STATLAWGVN 830 LPAHTVIIKG 840 TQVYSPEKGR 850 WTELGALDIL 860 QMLGRAGRPQ 870 YDTKGEGILI 880 TSHGELQYYL 890 SLLNQQLPIE 900 SQMVSKLPDM 910 LNAEIVLGNV 920 QNAKDAVNWL 930 GYAYLYIRML 940 RSPTLYGISH 950 DDLKGDPLLD 960 QRRLDLVHTA 970 ALMLDKNNLV 980 KYDKKTGNFQ 990 VTELGRIASH 1000 YYITNDTVQT 1010 YNQLLKPTLS 1020 EIELFRVFSL 1030 SSEFKNITVR 1040 EEEKLELQKL 1050 LERVPIPVKE 1060 SIEEPSAKIN 1070 VLLQAFISQL 1080 KLEGFALMAD 1090 MVYVTQSAGR 1100 LMRAIFEIVL 1110 NRGWAQLTDK 1120 TLNLCKMIDK 1130 RMWQSMCPLR 1140 QFRKLPEEVV 1150 KKIEKKNFPF 1160 ERLYDLNHNE 1170 IGELIRMPKM 1180 GKTIHKYVHL 1190 FPKLELSVHL 1200 QPITRSTLKV 1210 ELTITPDFQW 1220 DEKVHGSSEA 1230 FWILVEDVDS 1240 EVILHHEYFL 1250 LKAKYAQDEH 1260 LITFFVPVFE 1270 PLPPQYFIRV 1280 VSDRWLSCET 1290 QLPVSFRHLI 1300 LPEKYPPPTE 1310 LLDLQPLPVS 1320 ALRNSAFESL 1330 YQDKFPFFNP 1340 IQTQVFNTVY 1350 NSDDNVFVGA 1360 PTGSGKTICA 1370 EFAILRMLLQ 1380 SSEGRCVYIT 1390 PMEALAEQVY 1400 MDWYEKFQDR 1410 LNKKVVLLTG 1420 ETSTDLKLLG 1430 KGNIIISTPE 1440 KWDILSRRWK 1450 QRKNVQNINL 1460 FVVDEVHLIG 1470 GENGPVLEVI 1480 CSRMRYISSQ 1490 IERPIRIVAL 1500 SSSLSNAKDV 1510 AHWLGCSATS 1520 TFNFHPNVRP 1530 VPLELHIQGF 1540 NISHTQTRLL 1550 SMAKPVYHAI 1560 TKHSPKKPVI 1570 VFVPSRKQTR 1580 LTAIDILTTC 1590 AADIQRQRFL 1600 HCTEKDLIPY 1610 LEKLSDSTLK 1620 ETLLNGVGYL 1630 HEGLSPMERR 1640 LVEQLFSSGA 1650 IQVVVASRSL 1660 CWGMNVAAHL 1670 VIIMDTQYYN 1680 GKIHAYVDYP 1690 IYDVLQMVGH 1700 ANRPLQDDEG 1710 RCVIMCQGSK 1720 KDFFKKFLYE 1730 PLPVESHLDH 1740 CMHDHFNAEI 1750 VTKTIENKQD 1760 AVDYLTWTFL 1770 YRRMTQNPNY 1780 YNLQGISHRH 1790 LSDHLSELVE 1800 QTLSDLEQSK 1810 CISIEDEMDV 1820 APLNLGMIAA 1830 YYYINYTTIE 1840 LFSMSLNAKT 1850 KVRGLIEIIS 1860 NAAEYENIPI 1870 RHHEDNLLRQ 1880 LAQKVPHKLN 1890 NPKFNDPHVK 1900 TNLLLQAHLS 1910 RMQLSAELQS 1920 DTEEILSKAI 1930 RLIQACVDVL 1940 SSNGWLSPAL 1950 AAMELAQMVT 1960 QAMWSKDSYL 1970 KQLPHFTSEH 1980 IKRCTDKGVE 1990 SVFDIMEMED 2000 EERNALLQLT 2010 DSQIADVARF 2020 CNRYPNIELS 2030 YEVVDKDSIR 2040 SGGPVVVLVQ 2050 LEREEEVTGP 2060 VIAPLFPQKR 2070 EEGWWVVIGD 2080 AKSNSLISIK 2090 RLTLQQKAKV 2100 KLDFVAPATG 2110 AHNYTLYFMS 2120 DAYMGCDQEY 2130 KFSVDVKEAE TDSDSD

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0071013	catalytic step 2 spliceosome
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005681	spliceosomal complex
Cellular Component	GO:0071006	U2-type catalytic step 1 spliceosome
Cellular Component	GO:0071005	U2-type precatalytic spliceosome
Cellular Component	GO:0046540	U4/U6 x U5 tri-snRNP complex
Cellular Component	GO:0005682	U5 snRNP
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0004386	helicase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003724	RNA helicase activity
Biological Process	GO:0000354	cis assembly of pre-catalytic spliceosome
Biological Process	GO:0000398	mRNA splicing, via spliceosome
Biological Process	GO:0048025	negative regulation of mRNA splicing, via spliceosome
Biological Process	GO:0001649	osteoblast differentiation
Biological Process	GO:0000387	spliceosomal snRNP assembly
Biological Process	GO:0000244	spliceosomal tri-snRNP complex assembly
Biological Process	GO:0000388	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.