Search Results

Overview

Uniprot ID	O75746
Protein Name	Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial
Gene Name	SLC25A12
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
406	VAPEKAIKLTVNDFV
68	AGVADQTKDGLISYQ

Function

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:11566871, PubMed:19641205, PubMed:24515575, PubMed:38945283). Also mediates the uptake of L-cysteinesulfinate (3-sulfino-L-alanine) by mitochondria in exchange of L-glutamate and proton (PubMed:11566871). Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed:11566871). Lacks transport activity towards L-glutamine or gamma-aminobutyric acid (GABA) (PubMed:38945283)

Protein Sequence

10 MAVKVQTTKR 20 GDPHELRNIF 30 LQYASTEVDG 40 ERYMTPEDFV 50 QRYLGLYNDP 60 NSNPKIVQLL 70 AGVADQTKDG 80 LISYQEFLAF 90 ESVLCAPDSM 100 FIVAFQLFDK 110 SGNGEVTFEN 120 VKEIFGQTII 130 HHHIPFNWDC 140 EFIRLHFGHN 150 RKKHLNYTEF 160 TQFLQELQLE 170 HARQAFALKD 180 KSKSGMISGL 190 DFSDIMVTIR 200 SHMLTPFVEE 210 NLVSAAGGSI 220 SHQVSFSYFN 230 AFNSLLNNME 240 LVRKIYSTLA 250 GTRKDVEVTK 260 EEFAQSAIRY 270 GQVTPLEIDI 280 LYQLADLYNA 290 SGRLTLADIE 300 RIAPLAEGAL 310 PYNLAELQRQ 320 QSPGLGRPIW 330 LQIAESAYRF 340 TLGSVAGAVG 350 ATAVYPIDLV 360 KTRMQNQRGS 370 GSVVGELMYK 380 NSFDCFKKVL 390 RYEGFFGLYR 400 GLIPQLIGVA 410 PEKAIKLTVN 420 DFVRDKFTRR 430 DGSVPLPAEV 440 LAGGCAGGSQ 450 VIFTNPLEIV 460 KIRLQVAGEI 470 TTGPRVSALN 480 VLRDLGIFGL 490 YKGAKACFLR 500 DIPFSAIYFP 510 VYAHCKLLLA 520 DENGHVGGLN 530 LLAAGAMAGV 540 PAASLVTPAD 550 VIKTRLQVAA 560 RAGQTTYSGV 570 IDCFRKILRE 580 EGPSAFWKGT 590 AARVFRSSPQ 600 FGVTLVTYEL 610 LQRWFYIDFG 620 GLKPAGSEPT 630 PKSRIADLPP 640 ANPDHIGGYR 650 LATATFAGIE 660 NKFGLYLPKF 670 KSPSVAVVQP KAAVAATQ

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0000514	3-sulfino-L-alanine: proton, glutamate antiporter activity
Molecular Function	GO:0000515	aspartate:glutamate, proton antiporter activity
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0015183	L-aspartate transmembrane transporter activity
Molecular Function	GO:0005313	L-glutamate transmembrane transporter activity
Biological Process	GO:0015810	aspartate transmembrane transport
Biological Process	GO:0015813	L-glutamate transmembrane transport
Biological Process	GO:0043490	malate-aspartate shuttle
Biological Process	GO:0051592	response to calcium ion

Reference

[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[2] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.