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Overview

Uniprot IDO75832
Protein Name26S proteasome non-ATPase regulatory subunit 10
Gene NamePSMD10
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
30 KESILADKSLATRTD

Function

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis

Protein Sequence

10 MEGCVSNLMV 20 CNLAYSGKLE 30 ELKESILADK 40 SLATRTDQDS 50 RTALHWACSA 60 GHTEIVEFLL 70 QLGVPVNDKD 80 DAGWSPLHIA 90 ASAGRDEIVK 100 ALLGKGAQVN 110 AVNQNGCTPL 120 HYAASKNRHE 130 IAVMLLEGGA 140 NPDAKDHYEA 150 TAMHRAAAKG 160 NLKMIHILLY 170 YKASTNIQDT 180 EGNTPLHLAC 190 DEERVEEAKL 200 LVSQGASIYI 210 ENKEEKTPLQ 220 VAKGGLGLIL KRMVEG

Gene Ontology

Classification GO ID Description
Molecular Function GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005856 cytoskeleton
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000502 proteasome complex
Biological Process GO:0006915 apoptotic process
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
Biological Process GO:0043409 negative regulation of MAPK cascade
Biological Process GO:0090201 negative regulation of release of cytochrome c from mitochondria
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0030307 positive regulation of cell growth
Biological Process GO:0045737 positive regulation of cyclin-dependent protein serine/threonine kinase activity
Biological Process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Process GO:0031398 positive regulation of protein ubiquitination
Biological Process GO:0070682 proteasome regulatory particle assembly
Biological Process GO:0006357 regulation of transcription by RNA polymerase II

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.